The GenBank database contains over 2580 complete genome sequences from bacteriophages. However, limited reports are available concerning phages can that lyse members of Pseudomonas syringae, although this is a widespread bacterial species that can infect almost 200 plant species. In the present study, we isolated and characterized a new Siphoviridae phage, named "Pseudomonas phage vB_PsyS_Phobos" (for brevity, referred to here as Phobos). To our knowledge, this is one of the first genome sequences reported for a phage with lytic activity against P. syringae pv. syringae. The genome of Phobos is dsDNA of 56,734 bp with a GC content of 63.3%, containing 65 ORFs. Genome analysis revealed that Phobos is a novel lytic phage with unique genomic features and low similarity to other phages, suggesting that Phobos represents a new phage genus. Genome sequencing did not reveal sequences with significant similarity to known virulence factors, antibiotic resistance genes, potential immunoreactive allergens, or lysogeny-related proteins, suggesting suggests that phage Phobos is strictly lytic. Therefore, Phobos may be suitable for formulation as a biocontrol agent against P. syringae pv. syringae.

中文翻译：

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Phobos的完整基因组序列：一种新颖的噬菌体，具有异常的基因组特征，可感染丁香假单胞菌。

GenBank数据库包含来自噬菌体的2580多个完整基因组序列。然而，关于噬菌体可以溶解丁香假单胞菌的成员的报道很少，尽管这是一种广泛的细菌，可以感染近200种植物。在本研究中，我们分离并鉴定了一种新的名为假单胞菌噬菌体vB_PsyS_Phobos的幽门螺杆菌噬菌体（为简便起见，在这里称为Phobos）。据我们所知，这是报道的具有针对丁香假单胞菌pv的裂解活性的噬菌体的第一个基因组序列。丁香科。火卫一的基因组是56,734 bp的dsDNA，GC含量为63.3％，包含65个ORF。基因组分析表明，噬菌体是一种新颖的裂解噬菌体，具有独特的基因组特征，与其他噬菌体的相似性较低，这表明噬菌体代表了一种新的噬菌体。基因组测序未发现与已知毒力因子，抗生素抗性基因，潜在的免疫反应性变应原或溶原性相关蛋白具有显着相似性的序列，这表明噬菌体Phobos是严格裂解的。因此，Phobos可能适合用作抗丁香假单胞菌pv的生物防治剂。丁香科。