Mycobacterium abscessus subsp. massiliense (Mycma) belongs to the Mycobacterium abscessus complex and is a rapidly growing non-tuberculous mycobacterium. The chronic pulmonary, skin, and soft tissue infections that it causes may be difficult to treat due to its intrinsic resistance to the commonly used antimicrobial drugs, making it a serious world public health problem. Iron is an essential nutrient for the growth of microorganisms; nonetheless, it can be toxic when in excess. Thus, bacteria require an iron homeostasis mechanism to succeed in different environments. DNA-binding proteins from starved cells (Dps) are miniferritins with the property to act as additional iron storage proteins but also can bind to DNA, protecting it against hydroxyl radical. Annotation of the Mycma genome revealed the gene mycma_03135 with 79% sequential identity when compared to MSMEG_3242 gene from M. smegmatis mc2 155, which codifies for a known Dps. Recombinant Dps from M. abscessus (rMaDps) was produced in Escherichia coli, purified in soluble form and shown to form high mass oligomers in solution with ferroxidase activity, DNA binding, and protection against damage. The expression of the mycma_03135 gene was induced during Mycma growth in the presence of hydrogen peroxide (H2O2). Additionally, the expression of rMaDps by E. coli conferred greater resistance to H2O2. Thus, this study is the first to identify and characterize a Dps from M. abscessus. KEY POINTS: Mycobacterium abscessus subsp. massiliense express a miniferritin protein (Dps). Mycma Dps binds to DNA and protects against oxidative stress.

中文翻译：

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Dps蛋白与脓肿分枝杆菌亚种的抗性有关。应对压力大的状况。

脓肿分枝杆菌亚种 massiliense（Mycma）属于脓肿分枝杆菌复合体，是一种快速生长的非结核分枝杆菌。它引起的慢性肺，皮肤和软组织感染可能由于其对常用抗菌药物的内在抗性而难以治疗，这使其成为一个严重的世界公共卫生问题。铁是微生物生长的必需营养素。但是，过量使用会产生毒性。因此，细菌需要铁稳态机制才能在不同环境中成功。饥饿细胞（Dps）的DNA结合蛋白是微型铁蛋白，具有充当其他铁存储蛋白的功能，但也可以与DNA结合，从而保护其免受羟基自由基的侵害。Mycma基因组的注释显示，与来自耻垢分枝杆菌mc2 155的MSMEG_3242基因相比，该基因mycma_03135具有79％的序列同一性，该基因编码已知的Dps。脓肿分支杆菌的重组Dps（rMaDps）在大肠杆菌中产生，以可溶形式纯化，并显示在溶液中形成具有铁氧化酶活性，DNA结合和防止损坏的高品质低聚物。mycma_03135基因的表达是在过氧化氢（H2O2）存在下的Mycma生长过程中诱导的。此外，大肠杆菌表达rMaDps赋予了对H2O2的更大抵抗力。因此，这项研究是第一个鉴定和表征脓肿分支杆菌的Dps的研究。要点：脓肿分枝杆菌亚种。massiliense表达小铁蛋白（Dps）。Mycma Dps与DNA结合并防止氧化应激。