In this study, the metagenomic resource generated from an aquatic habitat of extreme temperature was screened for the identification of a novel xylanase, XynM1. Gene sequence analysis designated it as a member of glycoside hydrolase (GH) family 10. The metagenomic DNA fragment was cloned, expressed in Escherichia coli, and the purified protein was biochemically characterized. The optimum temperature and pH for the XynM1 xylanase were found to be at 80 °C and 7, respectively. It exhibited worthwhile pH stability by retaining about 70% activity in the range of pH 6 to 9 after the exposure for 12 h at 25 °C. Thermostability analysis established considerable heat tolerance in XynM1 protein at elevated temperatures, displaying about 50% residual activity after the exposure of 40 °C, 50 °C, 60 °C, and 70 °C for 20 h, 12 h, 6 h, and 1.5 h, respectively. The effects of additives such as metals, surfactants, and organic solvents were evaluated on the activity of XynM1. It was able to retain about 50% of its initial activity in the presence of NaCl concentration of 1 to 5 M. The novel xylanase was capable of hydrolyzing the hemicellulosic polymer, derived from diverse biomass sources, e.g., beechwood xylan, wheat arabinoxylan, corncob xylan, and sweet sorghum xylan. The XynM1-treated beechwood xylan manifested catalytic release of xylooligosaccharides (XOS) of 2-6 DP. The novel GH10 xylanase is a promising biocatalyst that could be ascribed for biomass conversion and production of prebiotic XOS biomolecules.

中文翻译：

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从极端高温温泉基因组中生产木寡糖的新型木聚糖酶的表征。

在这项研究中，筛选了从极端温度的水生生境中产生的宏基因组学资源，以鉴定新型木聚糖酶XynM1。基因序列分析将其指定为糖苷水解酶（GH）家族10的成员。克隆了宏基因组DNA片段，在大肠杆菌中表达，并对纯化的蛋白质进行了生化表征。发现XynM1木聚糖酶的最佳温度和pH分别为80°C和7。通过在25°C下暴露12 h后在pH 6到9的范围内保留约70％的活性，它具有值得的pH稳定性。热稳定性分析在高温下对XynM1蛋白建立了相当高的耐热性，在40°C，50°C，60°C和70°C暴露20 h，12 h，6 h和40°C后，显示出约50％的残留活性。分别为1.5小时。评估了诸如金属，表面活性剂和有机溶剂等添加剂对XynM1活性的影响。在NaCl浓度为1至5 M的情况下，它能够保留约50％的初始活性。新型木聚糖酶能够水解半纤维素聚合物，其衍生自多种生物质资源，例如山毛榉木聚糖，小麦阿拉伯木聚糖，玉米芯木聚糖和甜高粱木聚糖。XynM1处理的山毛榉木聚糖表现出2-6 DP的木寡糖（XOS）的催化释放。新型GH10木聚糖酶是一种很有前途的生物催化剂，可以归因于生物质转化和益生元XOS生物分子的生产。在NaCl浓度为1至5 M的情况下，它能够保留约50％的初始活性。新型木聚糖酶能够水解半纤维素聚合物，其衍生自多种生物质资源，例如山毛榉木聚糖，小麦阿拉伯木聚糖，玉米芯木聚糖和甜高粱木聚糖。XynM1处理的山毛榉木聚糖表现出2-6 DP的木寡糖（XOS）的催化释放。新型GH10木聚糖酶是一种很有前途的生物催化剂，可以归因于生物质转化和益生元XOS生物分子的生产。在NaCl浓度为1至5 M的情况下，它能够保留约50％的初始活性。新型木聚糖酶能够水解半纤维素聚合物，其衍生自多种生物质资源，例如山毛榉木聚糖，小麦阿拉伯木聚糖，玉米芯木聚糖和甜高粱木聚糖。XynM1处理的山毛榉木聚糖表现出2-6 DP的木寡糖（XOS）的催化释放。新型GH10木聚糖酶是一种很有前途的生物催化剂，可以归因于生物质转化和益生元XOS生物分子的生产。XynM1处理的山毛榉木聚糖表现出2-6 DP的木寡糖（XOS）的催化释放。新型GH10木聚糖酶是一种很有前途的生物催化剂，可以归因于生物质转化和益生元XOS生物分子的生产。XynM1处理的山毛榉木聚糖表现出2-6 DP的木寡糖（XOS）的催化释放。新型GH10木聚糖酶是一种很有前途的生物催化剂，可以归因于生物质转化和益生元XOS生物分子的生产。