The influence of probiotic supplementation on iron metabolism remains poorly investigated. However, a range of studies, especially on Lactobacillus plantarum 299v (Lp229v), have indicated a possible positive impact of probiotics on iron absorption. The aim of the study was to determine the effect of multistrain probiotic supply on iron balance. Thirty Wistar rats were randomized into three groups: placebo (KK group), and multistrain probiotic per os in a daily dose of 2.5 × 109 colony forming units (CFU) (PA group) or 1 × 1010 CFU (PB group). Multistrain probiotic consisted of nine bacterial strains: Bifidobacterium bifidum W23, B. lactis W51, B. lactis W52, Lactobacillus acidophilus W37, L. brevis W63, L. casei W56, L. salivarius W24, Lactococcus lactis W19, and Lc. lactis W58, in equal proportions. After six weeks, blood and organ samples were collected. No differences were found between the three groups in terms of serum concentrations of hepcidin (HEPC), lactoferrin (LTF), homocysteine (HCY), ferritin (Ft), or erythroferrone (ErFe), or in liver content of divalent metal transporter 1 (DMT1), transferrin receptors 1 and 2 (TfR), or ZRT/IRT-like protein 14 (ZIP14) proteins. In the overall sample, positive correlations were noted between the serum concentrations of hepcidin and lactoferrin, and hepcidin and ferritin; serum concentration of hepcidin and DMT1 and TfR1 in the liver; and serum concentration of erythroferrone and TfR2 in the liver. The correlations of serum hepcidin and erythroferrone with liver DMT1 and TfR represent significant mechanisms of Fe homeostasis. Our study has shown that multistrain probiotic supplementation used in the experiment did not disrupt the biochemical and hepatic regulatory processes of Fe balance and did not demonstrate significant influence on selected parameters of Fe metabolism.

中文翻译：

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Hepcidin 和 Erythroferrone 与补充多物种益生菌的大鼠的肝铁转运蛋白相关

益生菌补充剂对铁代谢的影响仍然缺乏研究。然而，一系列研究，特别是关于植物乳杆菌 299v (Lp229v) 的研究表明，益生菌可能对铁吸收产生积极影响。该研究的目的是确定多菌株益生菌供应对铁平衡的影响。30 只 Wistar 大鼠随机分为三组：安慰剂（KK 组）和多株益生菌口服，日剂量为 2.5 × 109 菌落形成单位（CFU）（PA 组）或 1 × 1010 CFU（PB 组）。Multistrain probiotic 由九种细菌菌株组成：双歧杆菌 W23、B.lactis W51、B.lactis W52、Lactobacillus acidophilus W37、L.brevis W63、L. casei W56、L. salivarius W24、Lactococcus lactis W19 和 Lc。乳 W58，等比例。六周后，收集了血液和器官样本。在铁调素 (HEPC)、乳铁蛋白 (LTF)、高半胱氨酸 (HCY)、铁蛋白 (Ft) 或赤铁酮 (ErFe) 的血清浓度或二价金属转运蛋白 1 的肝脏含量方面，三组之间没有发现差异（ DMT1)、转铁蛋白受体 1 和 2 (TfR) 或 ZRT/IRT 样蛋白 14 (ZIP14) 蛋白。在整个样本中，铁调素和乳铁蛋白的血清浓度与铁调素和铁蛋白的血清浓度呈正相关；肝脏中铁调素和 DMT1 和 TfR1 的血清浓度；以及肝脏中赤铁酮和 TfR2 的血清浓度。血清铁调素和赤铁酮与肝脏 DMT1 和 TfR 的相关性代表了 Fe 稳态的重要机制。