A mono-segmented sequential injection lab-at-valve (SI-LAV) system for the determination of albumin, glucose, and creatinine, three key biomarkers in diabetes screening and diagnosis, was developed as a single system for multi-analyte analysis. The mono-segmentation technique was employed for in-line dilution, in-line single-standard calibration, and in-line standard addition. This made adjustments to the sample preparation step easy unlike the batch-wise method. The results showed that the system could be used for both fast reaction (albumin) and slow reaction (glucose with enzymatic reaction and creatinine). In the case of slow reaction, the analysis time could be shortened by using the reaction rate obtained with the SI-LAV system. This proposed system is for cost-effective and downscaling analysis, which would be applicable for small hospitals and clinics in remote places with a small number of samples but relatively fast screening would be needed.

中文翻译：

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采用单段式流量系统的单段顺序进样阀门实验室测定白蛋白、葡萄糖和肌酐，可实现在线稀释、在线单标准校准和在线标准添加

用于测定白蛋白、葡萄糖和肌酐（糖尿病筛查和诊断中的三个关键生物标志物）的单段顺序注射阀门实验室 (SI-LAV) 系统被开发为用于多分析物分析的单一系统。单分段技术用于在线稀释、在线单标准校准和在线标准添加。与分批方法不同，这使得对样品制备步骤的调整变得容易。结果表明，该系统可用于快速反应（白蛋白）和慢反应（葡萄糖与酶促反应和肌酐）。在反应缓慢的情况下，可以通过使用 SI-LAV 系统获得的反应速率来缩短分析时间。这个提议的系统用于成本效益和缩小规模的分析，