Cellular functions rely on a series of organized and regulated multienzyme cascade reactions. The catalytic efficiencies of these cascades depend on the precise spatial organization of the constituent enzymes, which is optimized to facilitate substrate transport and regulate activities. Mimicry of this organization in a non-living, artificial system would be very useful in a broad range of applications—with impacts on both the scientific community and society at large. Self-assembled DNA nanostructures are promising applications to organize biomolecular components into prescribed, multidimensional patterns. In this review, we focus on recent progress in the field of DNA-scaffolded assembly and confinement of multienzyme reactions. DNA self-assembly is exploited to build spatially organized multienzyme cascades with control over their relative distance, substrate diffusion paths, compartmentalization and activity actuation. The combination of addressable DNA assembly and multienzyme cascades can deliver breakthroughs toward the engineering of novel synthetic and biomimetic reactors.

细胞功能依赖于一系列有组织和受调节的多酶级联反应。这些级联的催化效率取决于组成酶的精确空间组织，该组织经过优化以促进底物运输和调节活动。在非生命的人工系统中模拟这种组织将在广泛的应用中非常有用——对科学界和整个社会都有影响。自组装 DNA 纳米结构是将生物分子成分组织成规定的多维模式的有前途的应用。在这篇综述中，我们重点关注 DNA 支架组装和多酶反应限制领域的最新进展。利用 DNA 自组装来构建空间组织的多酶级联，并控制它们的相对距离、底物扩散路径、区室化和活性驱动。可寻址 DNA 组装和多酶级联的结合可以为新型合成和仿生反应器的工程带来突破。