Caloric reduction (CR) is considered as the most reasonable intervention to delay aging and age-related diseases. Numerous studies in various model organisms provide the main basis for this hypothesis. Human studies exist, but they differ widely in study design, characteristics of test persons and study outcome. In this study we investigated CR in humans on a molecular level to gain a better understanding in these processes. For that purpose, we analyzed human peripheral blood mononuclear cells of healthy people fasting according to F.X. Mayr. In a previous study our group could show a significantly improved DNA repair capacity after fasting. Here we were able to confirm these findings despite a slightly modified fasting therapy. Furthermore, the function of the mitochondrial respiratory chain and the mRNA levels of the mitochondria-associated genes SIRT3 and NDUFS1 were significantly affected by CR. However, these changes were only detectable in people who exhibited no improvement in DNA repair capacity. In contrast to that we could not observe any changes in ROS levels, mitochondrial DNA copy number and non-mitochondrial respiration. Altogether our results reveal that CR in form of F. X. Mayr therapy is able to positively influence several cellular parameters and especially mitochondrial function.

中文翻译：

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12 天的体内热量减少可以改善人类衰老的重要参数。

热量减少（CR）被认为是延缓衰老和年龄相关疾病最合理的干预措施。对各种模式生物的大量研究为这一假设提供了主要基础。存在人体研究，但它们在研究设计、受试者特征和研究结果方面差异很大。在这项研究中，我们在分子水平上研究了人类的 CR，以更好地了解这些过程。为此，我们根据 FX Mayr 分析了禁食健康人的人外周血单核细胞。在之前的一项研究中，我们的小组可以显示禁食后 DNA 修复能力显着提高。尽管对禁食疗法稍有改进，我们仍然能够证实这些发现。此外，线粒体呼吸链的功能和线粒体相关基因 SIRT3 和 NDUFS1 的 mRNA 水平受 CR 显着影响。然而，这些变化只能在 DNA 修复能力没有改善的人身上检测到。与此相反，我们无法观察到 ROS 水平、线粒体 DNA 拷贝数和非线粒体呼吸的任何变化。总而言之，我们的结果表明，FX Mayr 疗法形式的 CR 能够积极影响几个细胞参数，尤其是线粒体功能。线粒体 DNA 拷贝数和非线粒体呼吸。总而言之，我们的结果表明，FX Mayr 疗法形式的 CR 能够积极影响几个细胞参数，尤其是线粒体功能。线粒体 DNA 拷贝数和非线粒体呼吸。总而言之，我们的结果表明，FX Mayr 疗法形式的 CR 能够积极影响几个细胞参数，尤其是线粒体功能。