Combining the properties of natural protein and polysaccharide is a promising strategy to generate bioactive biomaterials with controlled structure. Here, a new method of preparing water-insoluble silk fibroin/hyaluronic acid (SF/HA) scaffolds with tunable performances using an all-aqueous process is reported. Freezing-induced assembly was used to form silk I crystallization in the SF/HA blends. Silk I crystallization enhanced the stability of SF/HA scaffolds in water by forming silk I crystal networks to entrap blended HA without chemical cross-linking. Increasing HA content significantly enhanced the flexibility and water binding capacity of porous scaffolds, but high amount of HA reduced the water-stability of porous scaffolds due to insufficient silk I crystal cross-links. The enzymatic degradation behavior of the SF/HA scaffolds was investigated, revealing that the regulation ability of HA in the SF scaffolds. This novel nonchemically cross-linked protein/polysaccharide scaffold may be useful for soft tissue engineering due to excellent biocompatibility and tunable performances.

中文翻译：

---

物理交联的丝素蛋白/透明质酸支架。

结合天然蛋白质和多糖的特性是产生具有受控结构的生物活性生物材料的有前途的策略。在此，报道了一种使用全水法制备具有可调性能的水不溶性丝素蛋​​白/透明质酸（SF / HA）支架的新方法。冷冻诱导的组装用于在SF / HA共混物中形成丝I结晶。Silk I结晶通过形成Silk I晶体网络以捕获混合的HA而没有化学交联，从而增强了SF / HA支架在水中的稳定性。HA含量的增加显着增强了多孔支架的柔韧性和水结合能力，但是大量的HA由于丝I晶体交联不足而降低了多孔支架的水稳定性。研究了SF / HA支架的酶促降解行为，揭示了HA在SF支架中的调节能力。这种新型的非化学交联的蛋白质/多糖支架由于出色的生物相容性和可调性能，可用于软组织工程。