Removal of malodorant skatole by two enriched microbial consortia: Performance, dynamic, function prediction and bacteria isolation.,Science of the Total Environment

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Removal of malodorant skatole by two enriched microbial consortia: Performance, dynamic, function prediction and bacteria isolation.
Science of the Total Environment ( IF 8.2 ) Pub Date : 2020-04-04 , DOI: 10.1016/j.scitotenv.2020.138416
Qiao Ma ₁ , Shengwei Liu ₁ , Shuzhen Li ₂ , Jiabao Hu ₁ , Minyi Tang ₁ , Yeqing Sun ₁

Affiliation

Malodor emission has become one of the major challenges in animal husbandry. Skatole, one of the most offensive odorous compounds, can cause several diseases to organisms and is resistant to biodegradation. However, the microbial community information for skatole degradation has yet to be reported. In this study, the aerobic sequencing batch reactors with two different inocula were constructed. Both Group N (sample from cattle house) and Group E (sample from goose house) could efficiently degrade skatole after 70 days operation under conditions of pH 7.0-9.0 and temperature 20-40 °C. High-throughput sequencing results showed that the α-diversity in Group N was higher than that in Group E, while neither of them changed during the whole operation process. Bacterial community structures in both groups shifted. Generally, Lactococcus, Pseudomonas and Flavobacterium remarkably reduced, while Arthrobacter became the dominant population. Function prediction results indicated that the xenobiotics biodegradation and metabolism category was significantly up-regulated in Group E but remained unchanged in Group N. On the other hand, culture-dependent technique was applied and ten bacteria were obtained from the sludges. Two strains belonged to Rhodococcus, a minor genus in the communities, were firstly proven to harbor excellent skatole-degrading capacity. This study proved that skatole could be effectively removed by activated sludges, and the non-core bacteria Rhodococcus would be functionally important in the degradation process. These findings provide new insights into our understanding of skatole biotransformation process, and offer valuable bacterial resources for bioremediation application.

中文翻译：

通过两个富集的微生物联盟去除恶臭臭臭臭素：性能，动态，功能预测和细菌分离。

恶臭排放已成为畜牧业的主要挑战之一。Skatole是最令人反感的臭味化合物之一，可对生物造成多种疾病，并具有抗生物降解性。但是，关于粪臭素降解的微生物群落信息尚未报道。在本研究中，构建了带有两个不同接种物的好氧排序批处理反应器。N组（来自牛舍的样品）和E组（来自鹅舍的样品）在pH 7.0-9.0和温度20-40°C的条件下运行70天后，都可以有效降解粪臭素。高通量测序结果表明，N组的α多样性高于E组，但在整个操作过程中均没有变化。两组的细菌群落结构发生了变化。通常，乳球菌假单胞菌和黄杆菌明显减少，而节杆菌成为主要种群。功能预测结果表明，异生物素的生物降解和代谢类别在E组中显着上调，但在N组中保持不变。另一方面，应用了依赖于培养的技术，并从污泥中获得了10种细菌。首先证实了两个属于红球菌属的菌种，该菌是该社区的次要属，具有出色的粪臭素降解能力。这项研究证明，活性污泥可以有效去除粪臭素，并且非核心细菌红球菌在降解过程中具有重要的功能。这些发现为我们对粪臭素生物转化过程的理解提供了新的见解，

更新日期：2020-04-06

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