Elevated bioaccumulation of PFAAs in Oryzias melastigma following the increase of salinity is associated with the up-regulated expression of PFAA-binding proteins.,Science of the Total Environment

当前位置： X-MOL 学术 › Sci. Total Environ. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Elevated bioaccumulation of PFAAs in Oryzias melastigma following the increase of salinity is associated with the up-regulated expression of PFAA-binding proteins.
Science of the Total Environment ( IF 8.2 ) Pub Date : 2020-04-03 , DOI: 10.1016/j.scitotenv.2020.138336
Ricardo David Avellán-Llaguno ₁ , Xiaobo Liu ₂ , Liangpo Liu ₃ , Sijun Dong ₄ , Qiansheng Huang ₄

Affiliation

Perfluoroalkyl acids (PFAAs) are widely detected in the environment, especially in estuarine and coastal areas where fluctuation of salinity occurs. Salinity alteration affected the distribution of PFAAs and even the bioaccumulation in organisms. However, the inner mechanism is still unclear. In this study, the marine medaka (Oryzias melastigma), a euryhaline fish model, was exposed to four PFAAs congeners under three different salinities (0, 15 and 35 psu). Results showed that the bioaccumulation of PFAAs increased in fish as the water salinity increased. PFAAs with longer lengths of carbon‑fluorine bond showed higher bioaccumulation in the fish. Salinity did not alter the levels of PFAAs in water media, however, the uptake rate of PFAAs from gills did increase with the salinity. Further analysis of the mechanism showed that PFAA bound to branchial proteins as confirmed by fluorescence spectroscopy. Higher expressions of proteins binding to PFAAs including organic anion transporter 1 (OAT1) and fatty acid-binding protein (FABP) facilitated the uptake of PFAAs through gills in fish culturing under higher salinity. In all, our study showed that elevation of salinity can induce the expression of proteins binding to PFAAs in gills, thus facilitate the uptake of water PFAAs. Salinity fluctuation should be taken into consideration when assessing the chemical risk in the estuarine and coastal areas.

中文翻译：

盐度增加后，M。Oryzias melastigma中PFAAs的生物蓄积增加与PFAA结合蛋白的表达上调有关。

全氟烷基酸（PFAAs）在环境中被广泛检测，尤其是在盐度发生波动的河口和沿海地区。盐度变化影响了PFAAs的分布，甚至影响了生物体内的生物积累。但是，内部机制仍不清楚。在这项研究中，海洋青aka（Oryzias melastigma）是一种淡淡的鱼类模型，在三种不同的盐度（0、15和35 psu）下暴露于四种PFAA同源物。结果表明，随着水盐度的增加，鱼类中PFAAs的生物积累增加。具有较长碳氟键长度的PFAAs在鱼类中显示出更高的生物蓄积性。盐度不会改变水介质中PFAA的含量，但是however的PFAA吸收率确实会随着盐度的增加而增加。对该机理的进一步分析表明，如荧光光谱所证实，PFAA与分支蛋白结合。结合至PFAA的蛋白质的较高表达，包括有机阴离子转运蛋白1（OAT1）和脂肪酸结合蛋白（FABP），有助于在较高盐度的鱼类养殖中通过g吸收PFAA。总之，我们的研究表明盐度的升高可以诱导g中与PFAAs结合的蛋白质表达，从而促进水PFAA的吸收。在评估河口和沿海地区的化学风险时，应考虑盐度波动。结合至PFAA的蛋白质的较高表达，包括有机阴离子转运蛋白1（OAT1）和脂肪酸结合蛋白（FABP），有助于在较高盐度的鱼类养殖中通过g吸收PFAA。总之，我们的研究表明盐度的升高可以诱导g中与PFAA结合的蛋白质表达，从而促进水PFAA的吸收。在评估河口和沿海地区的化学风险时，应考虑盐度波动。结合至PFAA的蛋白质的较高表达，包括有机阴离子转运蛋白1（OAT1）和脂肪酸结合蛋白（FABP），有助于在较高盐度的鱼类养殖中通过g吸收PFAA。总之，我们的研究表明盐度的升高可以诱导g中与PFAAs结合的蛋白质表达，从而促进水PFAA的吸收。在评估河口和沿海地区的化学风险时，应考虑盐度波动。

更新日期：2020-04-06

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11