Nitrate pollution presents a serious threat to the environment and public health. As an excellent heterotrophic denitrification carbon source, banana peel (a kind of agricultural waste) provides a feasible alternative to deal with the persistent high concentrations of nitrate pollution. Although the feasibility and economy of banana peel for denitrification have already been reported, the long-term stability and mechanism were still unclear. The coupling mechanism of organic matters and microorganism in the denitrification process was systematically investigated through a 17-cycle experiment. The results showed that significant NO₃⁻-N removal load and rate of 164.42 mg/g and 4.69 mg/(L·h) after long-term tests could be obtained. Organic matter analysis and 16S rRNA sequencing showed that the evolution of organic matter was dominated by Anaerolineaceae (fermenting bacteria), and, in the final step, the humification of organic matter was realized. Moreover, the presence of Lentimicrobium (denitrifying bacteria) was indispensable for the continuous removal of high concentrations of nitrate. The main functional gene of nitrogen transformation in this reaction system was NirS (haem-containing). This lab-scale heterotrophic denitrification process could contribute to a better understanding of the carbon and nitrogen cycles in the biogeochemical cycles to some extent, and it also provides a reference for the construction of highly efficient nitrate degradation reactors, based on agricultural wastes.

硝酸盐污染对环境和公众健康构成严重威胁。作为一种出色的异养反硝化碳源，香蕉皮（一种农业废弃物）为应对持续高浓度的硝酸盐污染提供了一种可行的替代方法。尽管已经报道了香蕉皮用于反硝化的可行性和经济性，但其长期稳定性和机理仍不清楚。通过17个循环的实验系统地研究了有机物与微生物在反硝化过程中的耦合机理。结果表明，显著NO ₃ ^-长期测试后，N去除量为164.42 mg / g和4.69 mg /（L·h）。有机物分析和16S rRNA测序表明，有机物的进化主要由厌氧菌（发酵细菌）主导，最后一步实现了有机物的腐殖化。此外，慢微生物的存在（反硝化细菌）对于连续去除高浓度的硝酸盐是必不可少的。该反应系统中氮转化的主要功能基因是NirS（含血红素）。这种实验室规模的异养反硝化过程可以在某种程度上有助于更好地理解生物地球化学循环中的碳和氮循环，并且还为基于农业废物的高效硝酸盐降解反应器的构建提供了参考。