The design of drugs from natural products is a re-emerging area due to the need for bioactive compounds. The exploitation of natural products and their derivatives obtained by biocatalysis is in line with the higher attention given today to new sustainable technologies that better preserve the environment (green chemistry). The research field of cytochromes P450 (CYPs) is continuously providing new enzymes and mutants that produce metabolites suitable for late-stage functionalization for new potential drugs. This review provides an overview of the exploitation of CYPs as biocatalysts in drug synthesis. Additionally, recent progress in protein and metabolic engineering is provided to show how these enzymes offer a toolbox that can be combined with other biocatalytic or chemical processes to build new platforms for the green production of new drugs.

中文翻译：

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天然化合物作为药物：细胞色素P450反应性的关键作用。

由于对生物活性化合物的需求，从天然产品设计药物成为一个新兴领域。通过生物催化获得的天然产物及其衍生物的开发与当今对更好地保护环境（绿色化学）的可持续技术的高度关注一致。细胞色素P450（CYPs）的研究领域正在不断提供新的酶和突变体，这些酶和突变体可产生适于新功能药物后期功能化的代谢产物。这篇综述概述了CYPs在药物合成中作为生物催化剂的开发。此外，提供了蛋白质和代谢工程学的最新进展，以显示这些酶如何提供可与其他生物催化或化学过程结合使用的工具箱，从而为绿色生产新药建立新的平台。