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Dietary nanoencapsulated quercetin homeostated transcription of redox-status orchestrating genes in zebrafish (Danio rerio) exposed to silver nanoparticles.
Environmental Research ( IF 7.7 ) Pub Date : 2020-04-06 , DOI: 10.1016/j.envres.2020.109477 Mohammad Behzadi Tayemeh 1 , Mohammad Reza Kalbassi 1 , Hamed Paknejad 2 , Hamid Salari Joo 1
Environmental Research ( IF 7.7 ) Pub Date : 2020-04-06 , DOI: 10.1016/j.envres.2020.109477 Mohammad Behzadi Tayemeh 1 , Mohammad Reza Kalbassi 1 , Hamed Paknejad 2 , Hamid Salari Joo 1
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The present study assessed the protective effect of chitosan-nanoencapsulated quercetin (Qu-ChiNPs) against oxidative stress caused by silver nanoparticles (AgNPs). To this end, the transcription of prime genes regulating hepatic Keap1-Nrf2 pathway as well as downstream antioxidant enzymes were monitored prior to and after oxidative stress by AgNPs. Zebrafish (Danio rerio; n = 225) was assigned into five experimental groups based on feeding with diets supplemented with different additives as follows: negative and positive control groups, without additive; ChiNPs, 400 mg nanochitosan per kg diet; Quercetin, 400 mg free quercetin per kg diet; and Qu-ChiNPs, 400 mg Qu-ChiNPs per kg diet. At the end of the feeding trial (40 days), the experimental groups, except the negative control, were exposed to sublethal concentration of AgNPs (0.15 mg L-1) for 96h. Before exposure to AgNPs, free quercetin-treated diet significantly upregulated Keap1, Nrf2, Cat, SOD, GPx, and GST genes in the liver tissue when compared with the control diet, whereas Qu-Chi.NPs downregulated their transcription to the lowest levels. After exposure to AgNPs, all genes exhibited different responses in the AgNPs-exposed groups. The highest transcription of Nrf2, Cat, SOD, GPx, and GST was observed in the positive group, with being upregulated about 8, 10, 8, 8, and 7 times, respectively, when compared to the respective ones in the negative control. However, Keap1 showed a reverse response with being transcripted 12 times lower. The quercetin treatments, especially Qu-Chi.NPs, significantly reduced the transcription of Nrf2, Cat, SOD, GPx, and GST genes, yet enhanced Keap1 expression. Qu-Chi.NPs reduced the expression of Nrf2, SOD, Cat, GPx, and GST about 11, 10, 15, 10, and 10 times, respectively, yet increased that of Keap1 about 12 times. Taken together, nanoencapsulation can improve the antioxidant efficacy of quercetin against AgNPs toxicity and might reduce involvement of the cellular antioxidant system through tuning redox status. More broadly, it would be interesting to assess the effects of Qu-Chi.NPs against other metallic and organic oxidative stressors or pollutants.
中文翻译：
膳食纳米封装的槲皮素使暴露于银纳米颗粒的斑马鱼（达尼奥里奥）中的氧化还原状态协调基因稳态转录。
本研究评估了壳聚糖纳米囊化槲皮素（Qu-ChiNPs）对银纳米颗粒（AgNPs）引起的氧化应激的保护作用。为此，在通过AgNPs氧化应激之前和之后，监测调节肝Keap1-Nrf2途径的主要基因以及下游抗氧化酶的转录。斑马鱼（Danio rerio； n = 225）根据饲喂补充了不同添加剂的日粮分为五个实验组：阴性和阳性对照组，无添加剂；ChiNPs，每公斤饮食400毫克纳米壳聚糖；槲皮素，每公斤饮食400毫克游离槲皮素；和Qu-ChiNPs，每公斤饮食400 mg Qu-ChiNPs。在喂养试验结束时（40天），除阴性对照组外，实验组均暴露于亚致死浓度的AgNPs（0。15 mg L-1）96小时。与对照饮食相比，游离槲皮素处理的饮食在暴露于AgNPs之前显着上调了肝组织中的Keap1，Nrf2，Cat，SOD，GPx和GST基因，而Qu-Chi.NPs将其转录下调至最低水平。暴露于AgNPs后，所有基因在暴露于AgNPs的组中表现出不同的响应。在阳性组中观察到Nrf2，Cat，SOD，GPx和GST的最高转录，与阴性对照中的相应转录分别分别上调约8、10、8、8和7倍。但是，Keap1显示出相反的反应，转录水平降低了12倍。槲皮素治疗，特别是Qu-Chi.NPs，显着降低Nrf2，Cat，SOD，GPx和GST基因的转录，但增强Keap1表达。曲智 NP分别使Nrf2，SOD，Cat，GPx和GST的表达降低约11、10、15、10和10倍，而使Keap1的表达提高约12倍。两者合计，纳米封装可以提高槲皮素抗AgNPs毒性的抗氧化功效，并可能通过调节氧化还原状态来减少细胞抗氧化系统的参与。更广泛地说，评估Qu-Chi.NPs对其他金属和有机氧化应激源或污染物的影响将是有趣的。
更新日期：2020-04-06
中文翻译：
膳食纳米封装的槲皮素使暴露于银纳米颗粒的斑马鱼（达尼奥里奥）中的氧化还原状态协调基因稳态转录。
本研究评估了壳聚糖纳米囊化槲皮素（Qu-ChiNPs）对银纳米颗粒（AgNPs）引起的氧化应激的保护作用。为此，在通过AgNPs氧化应激之前和之后，监测调节肝Keap1-Nrf2途径的主要基因以及下游抗氧化酶的转录。斑马鱼（Danio rerio； n = 225）根据饲喂补充了不同添加剂的日粮分为五个实验组：阴性和阳性对照组，无添加剂；ChiNPs，每公斤饮食400毫克纳米壳聚糖；槲皮素，每公斤饮食400毫克游离槲皮素；和Qu-ChiNPs，每公斤饮食400 mg Qu-ChiNPs。在喂养试验结束时（40天），除阴性对照组外，实验组均暴露于亚致死浓度的AgNPs（0。15 mg L-1）96小时。与对照饮食相比，游离槲皮素处理的饮食在暴露于AgNPs之前显着上调了肝组织中的Keap1，Nrf2，Cat，SOD，GPx和GST基因，而Qu-Chi.NPs将其转录下调至最低水平。暴露于AgNPs后，所有基因在暴露于AgNPs的组中表现出不同的响应。在阳性组中观察到Nrf2，Cat，SOD，GPx和GST的最高转录，与阴性对照中的相应转录分别分别上调约8、10、8、8和7倍。但是，Keap1显示出相反的反应，转录水平降低了12倍。槲皮素治疗，特别是Qu-Chi.NPs，显着降低Nrf2，Cat，SOD，GPx和GST基因的转录，但增强Keap1表达。曲智 NP分别使Nrf2，SOD，Cat，GPx和GST的表达降低约11、10、15、10和10倍，而使Keap1的表达提高约12倍。两者合计，纳米封装可以提高槲皮素抗AgNPs毒性的抗氧化功效，并可能通过调节氧化还原状态来减少细胞抗氧化系统的参与。更广泛地说，评估Qu-Chi.NPs对其他金属和有机氧化应激源或污染物的影响将是有趣的。
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