Nanosheets and nanosheet-based sorbents display great potential for adsorption and separation, but low recognition selectivity, the site loss caused by agglomeration and stacking, and difficulty in collection are the main bottlenecks for their practical application. In this work, the mosaic-inspired magnetic Alginate composite sorbents (J-SNs-MMIPs-Pickering) are prepared by combining Alg-Ca²⁺ Pickering emulsion with Janus MIPs nanosheets and superparamagnetic iron oxide nanoparticles, and then they are applied for selective separation of 2′-deoxyadenosine (dA). For this strategy, immobilized Janus MIPs nanosheets onto magnetic alginate composite act as stabilizer of Pickering emulsion and selective recognition sites for dA, and the magnetic responsibility is benefit for convenient collection from the sample solution. J-SNs-MMIPs-Pickering is expected to possess the advantages of excellent adsorption capacity, fast uptake kinetics, simple separation, and acceptable affinity towards dA. The equilibrium time and maximum adsorption capacity of J-SNs-MMIPs-Pickering are 1.0 h and 73.04 μmol g^-1 at 298 K, respectively, and the dispersed sorbents take about 30 s to be captured by the external magnetic field. By evaluating the selective adsorption of dA from spiked human urine and serum solutions, J-SNs-MMIPs-Pickering could be used for selective enrichment and analysis of targeted dA in complex biological samples. The present study not only provides insights to develop nanosheet-based sorbents by Pickering emulsion reactor, but also presents a new method for dA separation.

纳米片和基于纳米片的吸附剂显示出巨大的吸附和分离潜能，但识别选择性低，团聚和堆积引起的位点损失以及收集困难是其实际应用的主要瓶颈。在这项工作中，通过结合Alg-Ca ²⁺制备了镶嵌启发性的藻酸盐磁性复合吸附剂（J-SNs-MMIPs-Pickering）。用Janus MIPs纳米片和超顺磁性氧化铁纳米粒子进行Pickering乳液，然后将其用于2'-脱氧腺苷（dA）的选择性分离。对于此策略，将Janus MIPs纳米片固定在藻酸盐磁性复合材料上可充当Pickering乳液的稳定剂和dA的选择性识别位点，并且磁性作用有利于从样品溶液中方便收集。预计J-SNs-MMIPs-Pickering具有优异的吸附能力，快速的吸收动力学，简单的分离以及对dA可接受的亲和力的优点。J-SNs-MMIPs-Pickering的平衡时间和最大吸附容量为1.0 h和73.04μmolg ^-1分别在298 K时，分散的吸附剂大约需要30 s才能被外部磁场捕获。通过评估加标的人尿和血清溶液对dA的选择性吸附，J-SNs-MMIPs-Pickering可用于选择性富集和分析复杂生物样品中的目标dA。本研究不仅为利用Pickering乳液反应器开发基于纳米片的吸附剂提供了见识，而且为dA分离提供了一种新方法。