In the present work, for the first time, the filamentous fungus Fusarium sp. was utilized for devising a novel method for pre-concentration and determination of trace amounts of Pb(II), Cu(II), Cd(II), and Zn(II) ions, using a mini-column packed with Fusarium-coated multi-walled carbon nanotubes and inductively coupled plasma-optical emission spectrometry. Optimal analytical conditions including pH, ionic strength, elution solution, sample and eluent flow rates, and sample volume were determined. The detection limits were 0.39, 0.060, 0.021, and 0.025 ng mL-1 for Pb(II), Cu(II), Cd(II), and Zn(II) cations, respectively. This new method demonstrated a high performance for the analytes, and their adsorption was not affected by the different co-existing ions. The present procedure was validated by the analysis of standard reference materials, since the obtained data were in close agreement with reference values. Finally, this new procedure was successfully applied to analysis of heavy metal cations in natural food and water samples.

中文翻译：

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镰刀菌的应用 固定在多壁碳纳米管上，用于固相萃取和痕量分析重金属阳离子。

在目前的工作中，首次是丝状真菌镰刀菌。被用于设计一种新颖的方法，该方法使用填充有镰刀菌包被的多柱的微型柱来预富集和测定痕量的Pb（II），Cu（II），Cd（II）和Zn（II）离子壁碳纳米管和电感耦合等离子体发射光谱法。确定了最佳分析条件，包括pH，离子强度，洗脱液，样品和洗脱液的流速以及样品量。Pb（II），Cu（II），Cd（II）和Zn（II）阳离子的检出限分别为0.39、0.060、0.021和0.025 ng mL-1。这种新方法显示出对分析物的高性能，并且它们的吸附不受不同共存离子的影响。通过分析标准参考物质验证了本程序，因为获得的数据与参考值非常一致。最后，该新方法已成功应用于天然食品和水样中重金属阳离子的分析。