Detection of animal species in meat product is crucial to prevent adulterated and unnecessary contamination during processing. Gold standard is the real-time PCR assays, which can be conducted at highly equipped laboratories. Toward the development of point-of-need test, two rapid molecular assays based on recombinase polymerase amplification (RPA) for the detection of pork and horse DNA were established. Target genes are the porcine mitochondrial ND2 and equine ATP 6-8 genes. The pork and horse_RPA assays detected 16 and one DNA molecules/µl in eleven to six minutes, respectively. The myoglobin in the meat did not influence the assays performances, while the presence of high background-DNA induced a one log decrease in the sensitivity. Both assays are highly specific and identify down to 0.1% of their target DNA in meat mixtures. Both RPA assays could be used on-site as a rapid and mobile detection system to determine contamination of meat products.

中文翻译：

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重组酶聚合酶扩增法可鉴定猪肉和马肉。

肉类产品中动物种类的检测对于防止加工过程中的掺假和不必要的污染至关重要。金标准是实时PCR分析，可以在装备精良的实验室中进行。为了发展现场测试，建立了两种基于重组酶聚合酶扩增（RPA）的快速分子检测方法，用于检测猪肉和马的DNA。靶基因是猪线粒体ND2和马ATP 6-8基因。猪肉和马RPA分析在11至6分钟内分别检测到16和1个DNA分子/微升。肉中的肌红蛋白不影响测定性能，而高背景DNA的存在导致灵敏度下降了1个对数。两种检测方法均具有很高的特异性，可在肉类混合物中鉴定出低至目标DNA的0.1％。