Mass-produced Spirulina powder was characterized based on biomarkers for quality assessment. Other Spirulina powder products for functional foods and animal feeds were used as controls. In this study, Spirulina platensis was mass-cultured in modified Spirulina medium using a dispersive two-ton scale reactor for 30 days. After processing, the Spirulina powder was evaluated using FE-SEM and XPS. In the extracts, chlorophylls were determined using TLC and Q-TOF. SDS-PAGE and DSC were used to analyze protein biomarkers and to monitor thermal stability. The powder presented a microscale distorted sphere. Zinc, iron and calcium were detected on the powder surface. In the extracts, chlorophylls-a, -b, and -c, allophycocyanin, and phycocyanin-C were detected. Despite the similar morphology of all Spirulina products, the mass-produced Spirulina powder showed prolonged protein stability in biochemical compositions. The results suggest that mass-produced Spirulina powder can be characterized using biomarker-based advanced techniques. This protocol can be extended to other microalgae.

中文翻译：

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用于营养补充剂的高价值螺旋藻粉的大规模生产和基于生物标记的表征。


根据生物标志物对批量生产的螺旋藻粉进行表征，以进行质量评估。其他用于功能性食品和动物饲料的螺旋藻粉产品用作对照。在这项研究中，使用分散式两吨级反应器在改良螺旋藻培养基中对钝顶螺旋藻进行了 30 天的大规模培养。处理后，使用 FE-SEM 和 XPS 对螺旋藻粉末进行评估。使用 TLC 和 Q-TOF 测定提取物中的叶绿素。 SDS-PAGE 和 DSC 用于分析蛋白质生物标志物并监测热稳定性。该粉末呈现出微型扭曲的球体。在粉末表面检测到锌、铁和钙。在提取物中，检测到叶绿素-a、-b 和-c、别藻蓝蛋白和藻蓝蛋白-C。尽管所有螺旋藻产品的形态相似，但批量生产的螺旋藻粉末在生化成分中显示出延长的蛋白质稳定性。结果表明，可以使用基于生物标记的先进技术来表征批量生产的螺旋藻粉末。该协议可以扩展到其他微藻。