Neosporosis is caused by infection with the protozoa . It manifests as various neurological symptoms and is considered as one of the main causes of abortion in cattle, and induces uncommon congenital infection in sheep. The standard diagnosis is based on indirect immunofluorescence (IFI); however, cross-reactivity with other protozoa proteins is common. Aiming a more specific diagnosis, recombinant antigens have been tested in several immunoassays; of these, NcSAG1 (surface antigen-1) and NcSRS2 (SAG1-related sequence 2) were the most promising. In this context, we developed an indirect ELISA with recombinant NcSRS2 (ELISA-rNcSRS2) and NcSAG1 (ELISA-rNcSAG1) proteins alone and in association (ELISA-rNcSRS2/rNcSAG1) for the diagnosis of cattle and ovine neosporosis. A total of 216 samples from cattle and 154 samples from sheep were used to evaluate the ELISAs. The sensitivity and specificity results of the ELISA-rNcSRS2 were 91.5 % and 96.4 % for cattle, and 89.6 % and 96.3 % for sheep, respectively. For the ELISA-rNcSAG1, the sensitivity and specificity were 84.9 % and 97.3 % for cattle, and 89.6 % and 92.6 % for sheep, respectively. The sensitivity and specificity of the ELISA-rNcSRS2/rNcSAG1 was 98.1 % and 99.1 % for cattle, 100 % and 97.2 % for sheep, respectively. These results indicated that indirect ELISA using the rNcSRS2 and rNcSAG1 proteins are a highly sensitive and specific method, especially when used in association, for detecting antibodies in cattle and ovine populations infected with .

中文翻译：

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新孢子虫病的间接 ELISA：关联重组犬新孢子虫蛋白 NcSRS2 和 NcSAG1


新孢子虫病是由原虫感染引起的。它表现为各种神经系统症状，被认为是牛流产的主要原因之一，并引起绵羊罕见的先天性感染。标准诊断基于间接免疫荧光（IFI）；然而，与其他原生动物蛋白的交叉反应很常见。为了实现更具体的诊断，重组抗原已在多种免疫测定中进行了测试；其中，NcSAG1（表面抗原1）和NcSRS2（SAG1相关序列2）最有希望。在这种情况下，我们开发了一种单独使用重组 NcSRS2 (ELISA-rNcSRS2) 和 NcSAG1 (ELISA-rNcSAG1) 蛋白和联合 (ELISA-rNcSRS2/rNcSAG1) 蛋白的间接 ELISA，用于诊断牛和羊新孢子虫病。总共 216 个牛样本和 154 个绵羊样本用于评估 ELISA。 ELISA-rNcSRS2 的敏感性和特异性结果对牛分别为 91.5% 和 96.4%，对羊分别为 89.6% 和 96.3%。对于 ELISA-rNcSAG1，牛的敏感性和特异性分别为 84.9% 和 97.3%，羊的敏感性和特异性分别为 89.6% 和 92.6%。 ELISA-rNcSRS2/rNcSAG1 的灵敏度和特异性对于牛分别为 98.1 % 和 99.1 %，对于羊为 100 % 和 97.2 %。这些结果表明，使用 rNcSRS2 和 rNcSAG1 蛋白的间接 ELISA 是一种高度敏感和特异的方法，特别是在联合使用时，用于检测感染 .