Rapid and sensitive detection of prostate-specific antigen via label-free frequency shift Raman of sensing graphene.,Biosensors and Bioelectronics

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Rapid and sensitive detection of prostate-specific antigen via label-free frequency shift Raman of sensing graphene.
Biosensors and Bioelectronics ( IF 10.7 ) Pub Date : 2020-04-04 , DOI: 10.1016/j.bios.2020.112184
Sha Liu ₁ , Yapeng Huo ₂ , Jialei Bai ₃ , Baoan Ning ₃ , Yuan Peng ₃ , Shuang Li ₃ , Dianpeng Han ₃ , Weijun Kang ₄ , Zhixian Gao ₃

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The sensitive and accurate detection of cancer biomarkers is critically important to early clinical diagnosis, disease monitoring, and successful cancer treatment. Here, we first demonstrate an aptamer-based frequency shift Raman approach via sensing of graphene. This biosensor allows the rapid, sensitive, and label-free detection of the acknowledged protein cancer biomarker, prostate-specific antigen (PSA). Monolayer graphene is employed as the Raman substrate, which is highly sensitive to its electronic structure and interface properties. The PSA aptamer can be adsorbed strongly on the surface of substrates through π-π stacking interactions. The vibrational frequency of the G peak of graphene shifted upon the specific binding between the PSA and its aptamer. The corresponding frequency shifts of the G peak are directly correlated with PSA concentrations. The limit of detection is as low as 0.01 ng/mL, with a wide linear range from 0.05 ng/mL to 25 ng/mL. The analytic samples can be detected directly without any extensive preparation and label process. The whole detection is completed in only 30 min. Furthermore, excellent recoveries are acquired to validate the feasibility of this assay in human serum samples. The proposed technology could provide a selective, versatile, and user-friendly strategy for the early detection of cancer biomarkers.

中文翻译：

通过感应石墨烯的无标记移频拉曼快速而灵敏地检测前列腺特异性抗原。

敏感而准确地检测癌症生物标志物对于早期临床诊断，疾病监测和成功的癌症治疗至关重要。在这里，我们首先通过感测石墨烯展示基于适体的频移拉曼方法。这种生物传感器可以快速，灵敏且无标签地检测公认的蛋白癌症生物标志物，前列腺特异性抗原（PSA）。单层石墨烯被用作拉曼衬底，其对电子结构和界面特性高度敏感。PSA适体可以通过π-π堆积相互作用牢固地吸附在基材表面上。石墨烯G峰的振动频率随PSA及其适体之间的特异性结合而发生偏移。G峰的相应频移与PSA浓度直接相关。检测限低至0.01 ng / mL，线性范围从0.05 ng / mL到25 ng / mL。无需任何大量准备和标记过程即可直接检测分析样品。整个检测仅需30分钟即可完成。此外，获得了优异的回收率，以验证该测定在人血清样品中的可行性。所提出的技术可以为癌症生物标志物的早期检测提供一种选择性，通用和用户友好的策略。此外，获得了优异的回收率，以验证该测定在人血清样品中的可行性。所提出的技术可以为癌症生物标志物的早期检测提供一种选择性，通用和用户友好的策略。此外，获得了优异的回收率，以验证该测定在人血清样品中的可行性。所提出的技术可以为癌症生物标志物的早期检测提供一种选择性，通用和用户友好的策略。

更新日期：2020-04-06

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