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A novel fungal beta-propeller phytase from nematophagous Arthrobotrys oligospora: characterization and potential application in phosphorus and mineral release for feed processing
Microbial Cell Factories ( IF 4.3 ) Pub Date : 2020-04-06 , DOI: 10.1186/s12934-020-01346-9
Xianjuan Hou , Zhen Shen , Na Li , Xiaowei Kong , Kangliang Sheng , Jingmin Wang , Yongzhong Wang

Phytases are widely utilized in feed industry to increase the utilization of phosphorus, minerals, and amino acids for improvement of animal and human nutrition. At present, all known β-propeller phytases (BPP) have been generated from bacteria, particularly Bacillus. In this work we report for the first time a new fungal-derived BPP phytase. We identified a phytase highly differentially expressed only in the parasitic stage of a nematophagous fungus, Arhtrobotrys oliogospora, during the development of the 3D traps. We found that this phytase was homologous to the known bacterial BPP phytase, thus we referred the new phytase to Aophytase. The heterologous expression of codon-optimized Aophytase gene in Pichia pastoris was successfully investigated to yield recombinant Aophytase (r-Aophytase) with high specific enzyme activity of 74.71 U/mg, much higher than those of recombinant BPP phytases derived bacteria. The kinetic parameters of the r-Aophytase, the optimum pH and temperature, as well as the effects of surfactant, EDTA and different ions on its enzyme activity were further investigated. The potential utilization of r-Aophytase in feed processing was finally explored. We found that the optimal pH value was about 7.5, and the optimal temperature was 50 °C.; r-Aophytase significantly increased the release of inorganic phosphorus from soybean meal, and improved the release of soluble minerals from the durum wheat flour and finger millet flour. The findings indicate its potential utilization in the feed processing to ameliorate nutritional value of cereals and animal feed in the future.

中文翻译:

一种新的食虫性节肢动物的真菌β-螺旋植酸酶的特性及其在饲料加工过程中磷和矿物质释放中的应用

植酸酶广泛用于饲料工业,以提高磷,矿物质和氨基酸的利用率,从而改善动物和人类的营养。目前,所有已知的β-螺旋桨植酸酶(BPP)已经从细菌,特别是芽孢杆菌中产生。在这项工作中,我们首次报告了一种新的真菌来源的BPP植酸酶。我们确定了植酸酶在3D诱集装置的开发过程中仅在线虫真菌Arhtrobotrys oligogospora的寄生虫阶段高度差异表达。我们发现该植酸酶与已知的细菌BPP植酸酶同源,因此我们将新的植酸酶称为Aophytase。成功研究了密码子优化的Aophytase基因在巴斯德毕赤酵母中的异源表达,以产生具有74.71 U / mg高比酶活性的重组Aophytase(r-Aophytase),比重组BPP肌醇六磷酸酶衍生的细菌高得多。进一步研究了r-磷酸酶的动力学参数,最适pH和温度,以及表面活性剂,EDTA和不同离子对其酶活性的影响。最后探讨了r-磷酸酶在饲料加工中的潜在利用。我们发现最佳pH值约为7.5,最佳温度为50°C。r-磷酸酶显着增加了豆粕中无机磷的释放,并改善了硬质小麦粉和小米粉中可溶性矿物质的释放。研究结果表明,其未来在饲料加工中的潜在用途可改善谷物和动物饲料的营养价值。进一步研究了r-磷酸酶的动力学参数,最适pH和温度,以及表面活性剂,EDTA和不同离子对其酶活性的影响。最后探讨了r-磷酸酶在饲料加工中的潜在利用。我们发现最佳pH值约为7.5,最佳温度为50°C。r-磷酸酶显着增加了豆粕中无机磷的释放,并改善了硬质小麦粉和小米粉中可溶性矿物质的释放。研究结果表明,其未来在饲料加工中的潜在用途可改善谷物和动物饲料的营养价值。进一步研究了r-磷酸酶的动力学参数,最适pH和温度,以及表面活性剂,EDTA和不同离子对其酶活性的影响。最后探讨了r-磷酸酶在饲料加工中的潜在利用。我们发现最佳pH值约为7.5,最佳温度为50°C。r-磷酸酶显着增加了豆粕中无机磷的释放,并改善了硬质小麦粉和小米粉中可溶性矿物质的释放。研究结果表明,其未来在饲料加工中的潜在用途可改善谷物和动物饲料的营养价值。最后探讨了r-磷酸酶在饲料加工中的潜在利用。我们发现最佳pH值约为7.5,最佳温度为50°C。r-磷酸酶显着增加了豆粕中无机磷的释放,并改善了硬质小麦粉和小米粉中可溶性矿物质的释放。研究结果表明,其未来在饲料加工中的潜在用途可改善谷物和动物饲料的营养价值。最后探讨了r-磷酸酶在饲料加工中的潜在利用。我们发现最佳pH值约为7.5,最佳温度为50°C。r-磷酸酶显着增加了豆粕中无机磷的释放,并改善了硬质小麦粉和小米粉中可溶性矿物质的释放。研究结果表明,其未来在饲料加工中的潜在用途可改善谷物和动物饲料的营养价值。
更新日期:2020-04-22
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