Tracking Subtle Membrane Disruptions with a Tethered Photoacid,ChemPhotoChem

当前位置： X-MOL 学术 › ChemPhotoChem › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Tracking Subtle Membrane Disruptions with a Tethered Photoacid
ChemPhotoChem ( IF 3.0 ) Pub Date : 2020-05-07 , DOI: 10.1002/cptc.202000024
Nadav Amdursky ₁ , Yiyang Lin ₂

Affiliation

Fluorescent dyes are common tools for tracking biological membranes. In this work, we introduce a new ratiometric fluorescent probe for membranes based on a surface‐sensitive photoacid. As all other probes, it lights up within membranes, and furthermore, it has different ratios of fluorescent peaks for different membranes. The main unique feature of the probe is its ability to sense events taking place in the membrane far away from its actual location. This is due to the photoacidity of the probe, and the associated kinetics of its excited‐state proton release and capture that are highly sensitive to the lateral proton diffusion on the surface of the membrane. We show here that minor disruptions to the membrane integrity, induced by the Triton X‐100 surfactant at low concentration (surfactant:lipid ratio of 0.02), can be sensed by our probe using steady‐state fluorescence, while other probes are invisible to them. Time‐resolved fluorescence reveals that the surfactant insertion affects the lateral proton diffusion and accordingly the measured proton recombination of the photoacid.

中文翻译：

用束缚光酸跟踪细微的膜破坏

荧光染料是追踪生物膜的常用工具。在这项工作中，我们介绍了一种基于表面敏感光酸的新型比率式荧光膜探针。与所有其他探针一样，它在膜内也会亮起，此外，对于不同的膜，它具有不同的荧光峰比率。探头的主要独特功能是它能够感知远离实际位置的膜中发生的事件。这是由于探针的光酸度，以及其激发态质子释放和捕获的相关动力学，这些动力学对质子在膜表面的横向扩散非常敏感。我们在这里表明，在低浓度（表面活性剂：脂质比为0.02）下，Triton X-100表面活性剂会引起膜完整性的轻微破坏，我们的探头可以使用稳态荧光来感应，而其他探头对它们是不可见的。时间分辨荧光表明表面活性剂的插入会影响质子的横向扩散，从而影响光酸的质子重组。

更新日期：2020-05-07

点击分享查看原文

点击收藏

阅读更多本刊最新论文