The antibacterial properties of self-cleaning coatings are based on bactericide nanoparticles (NPs). Ecotoxicity of these NPs have been assessed mostly in suspension, using standard bioassays. Here a protocol is proposed to test actual coating samples, using the Vibrio fischeri bioluminescence inhibition bioassay. The protocol was designed to test bactericide properties of specially coated PVC floors being used in hospital environments under quasinatural conditions, such as prolonged exposure or room temperature. To take into consideration that the light output of the bacteria under prolonged exposure naturally changes, a correction factor is proposed.

中文翻译：

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自我清洁地板的潜在杀菌作用评估：拟议方案。

自清洁涂料的抗菌性能基于杀菌剂纳米颗粒（NPs）。这些NP的生态毒性主要是使用标准生物测定法在悬浮液中评估的。在此，提出了使用费氏弧菌生物发光抑制生物测定法测试实际涂层样品的方案。该协议旨在测试在准自然条件下（例如长时间暴露或室温）在医院环境中使用的特殊涂层PVC地板的杀菌性能。考虑到长时间暴露下细菌的光输出自然变化，提出了校正因子。