Nuclear Mechanics within Intact Cells Is Regulated by Cytoskeletal Network and Internal Nanostructures.,Small

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Nuclear Mechanics within Intact Cells Is Regulated by Cytoskeletal Network and Internal Nanostructures.
Small ( IF 13.0 ) Pub Date : 2020-04-03 , DOI: 10.1002/smll.201907688
Jitao Zhang ₁ , Farid Alisafaei _{2,

3} , Miloš Nikolić ₄ , Xuefei A Nou ₁ , Hanyoup Kim ₅ , Vivek B Shenoy _{2,

3} , Giuliano Scarcelli _{1,

4}

Affiliation

The mechanical properties of the cellular nucleus are extensively studied as they play a critical role in important processes, such as cell migration, gene transcription, and stem cell differentiation. While the mechanical properties of the isolated nucleus have been tested, there is a lack of measurements about the mechanical behavior of the nucleus within intact cells and specifically about the interplay of internal nuclear components with the intracellular microenvironment, because current testing methods are based on contact and only allow studying the nucleus after isolation from a cell or disruption of cytoskeleton. Here, all-optical Brillouin microscopy and 3D chemomechanical modeling are used to investigate the regulation of nuclear mechanics in physiological conditions. It is observed that the nuclear modulus can be modulated by epigenetic regulation targeting internal nuclear nanostructures such as lamin A/C and chromatin. It is also found that nuclear modulus is strongly regulated by cytoskeletal behavior through a robust mechanism conserved in different culturing conditions. Given the active role of cytoskeletal modulation in nearly all cell functions, this work will enable to reveal highly relevant mechanisms of nuclear mechanical regulations in physiological and pathological conditions.

中文翻译：

完整细胞内的核力学受到细胞骨架网络和内部纳米结构的调节。

细胞核的机械特性被广泛研究，因为它们在细胞迁移、基因转录和干细胞分化等重要过程中发挥着关键作用。虽然已经测试了分离细胞核的机械性能，但缺乏对完整细胞内细胞核机械行为的测量，特别是对内部核成分与细胞内微环境相互作用的测量，因为当前的测试方法基于接触并且仅允许在与细胞分离或细胞骨架破坏后研究细胞核。在这里，全光学布里渊显微镜和 3D 化学力学模型用于研究生理条件下核力学的调节。据观察，核模量可以通过针对内部核纳米结构（例如核纤层蛋白 A/C 和染色质）的表观遗传调控来调节。还发现核模量通过在不同培养条件下保守的稳健机制受到细胞骨架行为的强烈调节。鉴于细胞骨架调节在几乎所有细胞功能中的积极作用，这项工作将能够揭示生理和病理条件下核机械调节的高度相关机制。

更新日期：2020-04-03

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