ABSTRACT In this study, a monoclonal antibody with selectivity for clopidol was produced based on a new hapten. The 50% inhibition concentration (IC50) for the developed ic-ELISA was 0.275 ng/mL. Cross-reactivity tests showed that the monoclonal antibody had high specificity for clopidol. Using this antibody, a lateral-flow colloidal gold immunoassay (LCGA) was created for the rapid screening of clopidol in raw chicken samples. With the naked eye, the cut-off value of the strip assay was 5 μg/kg for clopidol in chicken samples. Therefore, this LCGA provides a sensitive and rapid method for on-site detection of clopidol in chicken samples.

中文翻译：

---

基于金纳米颗粒的试纸条检测鸡中氯吡多的开发

摘要 在本研究中，基于一种新的半抗原制备了一种对氯吡醇具有选择性的单克隆抗体。开发的 ic-ELISA 的 50% 抑制浓度 (IC50) 为 0.275 ng/mL。交叉反应试验表明该单克隆抗体对氯吡多具有高度特异性。使用这种抗体，创建了侧流胶体金免疫分析 (LCGA)，用于快速筛选生鸡肉样品中的氯吡多。肉眼观察，鸡样本中氯吡多的条带检测临界值为 5 μg/kg。因此，该LCGA为鸡肉样品中氯吡醇的现场检测提供了一种灵敏、快速的方法。