ABSTRACT In this study, we produced a monoclonal antibody (mAb) 2F7 against cadmium (Cd) with IC50 of 0.20 ng/mL. The cross-reactivity of mAb 2F7 with other eight metals was < 1%. A fluorescent immunoassay strip was developed for the determination of Cd in rice. The cut-off value by visual inspection of the test strip was 50 μg/kg, and the quantitative detection range determined by the strip reader was 3.86–48.92 μg/kg. Both ic-ELISA and the fluorescent immunoassay strip can be used to detect Cd rice. Therefore, our developed fluorescent immunoassay strip may be an effective tool for detecting Cd in rice.

中文翻译：

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水稻中镉的快速定量检测荧光免疫检测试纸条的研制

摘要 在本研究中，我们生产了一种抗镉 (Cd) 的单克隆抗体 (mAb) 2F7，IC50 为 0.20 ng/mL。mAb 2F7 与其他八种金属的交叉反应性小于 1%。开发了一种用于测定水稻中 Cd 的荧光免疫测定条。试纸目测截断值为50 μg/kg，条形阅读器测定的定量检测范围为3.86-48.92 μg/kg。ic-ELISA 和荧光免疫检测试纸条均可用于检测 Cd 水稻。因此，我们开发的荧光免疫检测试纸条可能是检测水稻中镉的有效工具。