Abstract By using the π-electron rich 4,4′-stilbenedicarboxylic acid (H2sda), a Zn(II)-bearing metal-organic framework with the chemical formula of [Zn3(sda)3(H2O)2](H2O)4 (1) has been prepared through the reaction of the 4,4′-stilbenedicarboxylic acid with Zn(NO3)2·6H2O in the component solvent of H2O and MeOH with aid of HBF4. Considering its intensive fluorescent emission, 1 was studied as a fluorescent sensor for metal ions in water, and the results reveal that 1 can be developed into an excellent Pb2+ sensor in water by utilizing luminescence quenching with a detection limit of 32.6 nm. In biological research, we explored the protective effect of 1 in OA rats. First, the DCFH-DA staining assay was utilized to detect the ROS levels in primary chondrocyte. Then, the Annexin V-FITC/PI was conducted for evaluating the apoptotic cells percentage after treatment with 1. The results from molecular docking software exhibited a possible mode of the compound binding to Bcl2, indicating a potential modulation mechanism. Graphical Abstract

中文翻译：

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用于选择性检测 Pb2+ 的荧光 Zn(II)-有机框架和通过减少软骨细胞中 ROS 积累和凋亡在骨关节炎治疗中的保护作用

摘要 通过使用富 π 电子 4,4'-芪二甲酸 (H2sda)，一种含 Zn(II) 的金属有机骨架，化学式为 [Zn3(sda)3(H2O)2](H2O)4 (1) 是通过 4,4'-芪二甲酸与 Zn(NO3)2·6H2O 在 H2O 和 MeOH 的组分溶剂中借助 HBF4 反应制备的。考虑到其强烈的荧光发射，1 被研究作为水中金属离子的荧光传感器，结果表明 1 可以利用检测限为 32.6 nm 的发光淬灭开发成一种优良的水中 Pb2+ 传感器。在生物学研究中，我们探索了 1 对 OA 大鼠的保护作用。首先，利用 DCFH-DA 染色法检测原代软骨细胞中的 ROS 水平。然后，Annexin V-FITC/PI 用于评估用 1 处理后的凋亡细胞百分比。分子对接软件的结果显示化合物与 Bcl2 结合的可能模式，表明潜在的调节机制。图形概要