Light guidance is a convenient and versatile way to control the positions of phototactic microorganisms. However, the illumination strategies require adaption to the respective organism. We report on the generation of structures composed of the gliding and exopolysaccharide-secreting algae Porphyridium purpureum via their photomovement. Light patterns from a two-dimensional computer-generated hologram were projected onto inoculated agar plates. The obtained pixelated algae patterns were evaluated with regard to the illuminated intensity, contrast and pixel size. Upper and lower thresholds for algae accumulation were determined, allowing to enhance future manipulation of phototactic microorganisms.

中文翻译：

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紫菜紫菜的光衍射图案。

光导是控制光战术微生物位置的方便且通用的方法。但是，照明策略需要适应相应的有机体。我们报道了通过滑行和分泌多糖的藻类紫藻紫罗兰的光运动组成的结构的生成。来自二维计算机生成的全息图的光图案投射到接种的琼脂平板上。关于照明强度，对比度和像素尺寸，评估获得的像素化藻类图案。确定了藻类积累的上限和下限，从而可以增强对光战术微生物的进一步控制。