The characterization of self-assembled nanostructures in whole blood,Analytical Methods

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The characterization of self-assembled nanostructures in whole blood
Analytical Methods ( IF 2.7 ) Pub Date : 2020-04-03 , DOI: 10.1039/d0ay00170h
Mark Pitman _{1,

2,

3,

4} , Jessica Larsen _{1,

2,

3,

4,

5}

Affiliation

Nanomedicine and targeted drug delivery systems hold significant promise, but suffer from a lack of successful translation into clinical use. A major factor is the inability to properly characterize important aspects of drug delivery systems in a realistic environment in vitro. Self-assembled particles are of particular concern because aqueous solutions may have different forces contributing to assembly, compared to the complex fluids inside the body. Characterization techniques for nanoparticles, the theory behind the techniques, and their shortcomings for use with blood are surveyed. Previous attempts to replicate or model blood for use with these techniques are examined. The complexity of blood, both its non-Newtonian nature and highly polydisperse cell and protein populations, makes it difficult to reliably study in situ nanoparticle properties in a way that resembles in vivo behavior. However, comparatively little focus is given to this issue: studies frequently use unreliable models of whole blood or jump from aqueous solutions directly to animal models, which can limit translational success. New technologies and methods to study nanoparticles in blood could provide valuable insight into how delivery systems will function in practice, and help to find promising candidates to move into animal or clinical trials.

中文翻译：

全血中自组装纳米结构的表征

纳米医学和靶向药物递送系统具有巨大的前景，但缺乏成功转化为临床用途的困扰。一个主要因素是无法在现实的体外环境中正确表征药物输送系统的重要方面。自组装颗粒是特别值得关注的，因为与体内复杂的流体相比，水溶液可能具有不同的作用于组装的力。研究了纳米粒子的表征技术，该技术背后的理论及其与血液一起使用的缺点。检查了以前复制或建模血液以与这些技术一起使用的尝试。血液的复杂性，既是非牛顿性的，又是高度分散的细胞和蛋白质，这使得难以像在体内一样可靠地研究原位纳米粒子的性质。行为。但是，对此问题的关注相对较少：研究经常使用不可靠的全血模型或从水溶液直接跳转到动物模型，这可能会限制翻译成功。研究血液中纳米颗粒的新技术和方法可以提供宝贵的见解，以了解输送系统在实践中将如何发挥作用，并有助于寻找有前途的候选对象，以进行动物或临床试验。

更新日期：2020-04-03

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