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GMBP1-conjugated manganese oxide nanoplates for in vivo monitoring of gastric cancer MDR using magnetic resonance imaging
RSC Advances ( IF 3.9 ) Pub Date : 2020-4-3 , DOI: 10.1039/d0ra00897d Wenhua Zhan 1, 2 , Xiaoxia Cai 3 , Hairui Li 3 , Getao Du 3 , Hao Hu 4 , Yayan Wu 1 , Lin Wang 5
RSC Advances ( IF 3.9 ) Pub Date : 2020-4-3 , DOI: 10.1039/d0ra00897d Wenhua Zhan 1, 2 , Xiaoxia Cai 3 , Hairui Li 3 , Getao Du 3 , Hao Hu 4 , Yayan Wu 1 , Lin Wang 5
Affiliation
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Multidrug resistance (MDR) is a huge challenge for gastric cancer chemotherapy. Therefore, MDR accurate monitoring is of great significance for the treatment of gastric cancer. GMBP1, an extracellular internalization peptide, can target MDR gastric cancer cells through specific binding to GRP78, which is an MDR-related protein that is overexpressed in gastric cancer cells. Herein, we constructed GMBP1 conjugated Mn3O4 nanoplates (Mn3O4@PEG-GMBP1 NPs) for in vivo monitoring of MDR gastric cancer through magnetic resonance imaging (MRI). The generated Mn3O4@PEG-GMBP1 NPs had a size of about 11 nm and exhibited a good colloidal stability in PBS and in 10% FBS medium. Serial in vivo MRI studies in mice demonstrated that the magnetic resonance signal intensity, at the tumor site, reached a peak at 3 h after tail vein injection of Mn3O4@PEG-GMBP1 NPs. The specific targeting ability of MDR gastric cancer cells (SGC7901/ADR) by Mn3O4@PEG-GMBP1 NPs was authenticated in vitro, in vivo and by immunofluorescence analysis experiments. The systematic safety evaluation indicated that the toxicity of Mn3O4@PEG-GMBP1 NPs in mice was negligible. Therefore, the GMBP1 conjugated Mn3O4 nanoplates can be clinically used for accurate imaging and monitoring of MDR gastric cancer.
中文翻译：
GMBP1-共轭氧化锰纳米板用于使用磁共振成像在体内监测胃癌 MDR
多药耐药（MDR）是胃癌化疗面临的巨大挑战。因此，MDR准确监测对于胃癌的治疗具有重要意义。GMBP1是一种细胞外内化肽，可通过特异性结合GRP78靶向MDR胃癌细胞，GRP78是一种在胃癌细胞中过表达的MDR相关蛋白。在此，我们构建了 GMBP1 共轭 Mn 3 O 4纳米板 (Mn 3 O 4 @PEG-GMBP1 NPs)，用于通过磁共振成像 (MRI)在体内监测 MDR 胃癌。生成的 Mn 3 O 4@PEG-GMBP1 NPs 的大小约为 11 nm，在 PBS 和 10% FBS 培养基中表现出良好的胶体稳定性。小鼠体内连续MRI 研究表明，肿瘤部位的磁共振信号强度在尾静脉注射 Mn 3 O 4 @PEG-GMBP1 NPs 后 3 小时达到峰值。通过体外、体内和免疫荧光分析实验验证了Mn 3 O 4 @PEG-GMBP1 NPs对MDR胃癌细胞（SGC7901/ADR）的特异性靶向能力。系统的安全性评价表明，Mn 3 O 4的毒性小鼠中的@PEG-GMBP1 NPs 可以忽略不计。因此，GMBP1共轭Mn 3 O 4纳米片可在临床上用于MDR胃癌的准确成像和监测。
更新日期：2020-04-03
中文翻译：
GMBP1-共轭氧化锰纳米板用于使用磁共振成像在体内监测胃癌 MDR
多药耐药（MDR）是胃癌化疗面临的巨大挑战。因此，MDR准确监测对于胃癌的治疗具有重要意义。GMBP1是一种细胞外内化肽，可通过特异性结合GRP78靶向MDR胃癌细胞，GRP78是一种在胃癌细胞中过表达的MDR相关蛋白。在此，我们构建了 GMBP1 共轭 Mn 3 O 4纳米板 (Mn 3 O 4 @PEG-GMBP1 NPs)，用于通过磁共振成像 (MRI)在体内监测 MDR 胃癌。生成的 Mn 3 O 4@PEG-GMBP1 NPs 的大小约为 11 nm，在 PBS 和 10% FBS 培养基中表现出良好的胶体稳定性。小鼠体内连续MRI 研究表明，肿瘤部位的磁共振信号强度在尾静脉注射 Mn 3 O 4 @PEG-GMBP1 NPs 后 3 小时达到峰值。通过体外、体内和免疫荧光分析实验验证了Mn 3 O 4 @PEG-GMBP1 NPs对MDR胃癌细胞（SGC7901/ADR）的特异性靶向能力。系统的安全性评价表明，Mn 3 O 4的毒性小鼠中的@PEG-GMBP1 NPs 可以忽略不计。因此，GMBP1共轭Mn 3 O 4纳米片可在临床上用于MDR胃癌的准确成像和监测。
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