Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Colorimetric sensing of chlorpyrifos through negative feedback inhibition of the catalytic activity of silver phosphate oxygenase nanozymes
RSC Advances ( IF 3.9 ) Pub Date : 2020-4-1 , DOI: 10.1039/c9ra10719c Amisha Kushwaha 1 , Gajendar Singh 1 , Manu Sharma 1
RSC Advances ( IF 3.9 ) Pub Date : 2020-4-1 , DOI: 10.1039/c9ra10719c Amisha Kushwaha 1 , Gajendar Singh 1 , Manu Sharma 1
Affiliation
|
Intensive use of organophosphate chlorpyrifos pesticides in farming has become a serious issue due to their harmful effects on living beings. Most fruits, vegetables and soil contain chlorpyrifos, and it cannot be rinsed out completely by water washing. Therefore, a selective and sensitive detection of chlorpyrifos is significant. In the present study, the intriguing oxidase-mimicking activity of Ag3PO4 nanoparticles (NPs) is explored for the fast and selective detection of chlorpyrifos pesticides. Ag3PO4 NPs exhibit several advantages, such as great catalytic efficiency, high stability, monodispersity and reusability, over other expensive nanozymes via a facile one-step sensing. The size, shape, crystal planes and diffraction patterns of the Ag3PO4 NPs were observed via FESEM and HR-TEM. The surface properties and oxidation states were analyzed via XPS technique. Ag3PO4 NPs possess intrinsic excellent oxidase-mimicking properties against 3,3′,5,5′-tetramethylbezidyne (TMB). When chlorpyrifos and Ag3PO4 NP nanozymes come in proper orientation proximity, chlorpyrifos is oxidized. The oxidized chlorpyrifos produces sulfide ions and chlorpyrifos oxon. The produced sulfide ions in the reaction system interact with Ag3PO4 NPs and inhibit their catalytic activity by feedback inhibition. Indeed, neither any catalytic site is left to oxidize TMB nor any blue colour appears. Thus, this feedback inhibition phenomenon senses chlorpyrifos pesticides. The calculated limit of detection (LOD) for the standard chlorpyrifos is ∼9.97 ppm, and the efficacy of the Ag3PO4 NPs calculated in terms of the Km value was found to be 0.15 mM. A real sample analysis was carried out by the standard addition method with two soil samples collected from Pethapur and Chiloda villages.
中文翻译：
通过负反馈抑制磷酸银加氧酶纳米酶的催化活性比色传感毒死蜱
有机磷毒死蜱农药在农业中的大量使用因其对生物的有害影响已成为一个严重的问题。大多数水果、蔬菜和土壤都含有毒死蜱，用水洗不能完全冲洗掉。因此，毒死蜱的选择性和灵敏检测意义重大。在本研究中，探索了 Ag 3 PO 4纳米粒子 (NPs) 的耐人寻味的氧化酶模拟活性，用于快速和选择性地检测毒死蜱农药。与其他昂贵的纳米酶相比， Ag 3 PO 4 NPs 具有催化效率高、稳定性高、单分散性和可重复使用性等优点。一种简单的一步传感。通过FESEM和HR-TEM观察Ag 3 PO 4 NPs的尺寸、形状、晶面和衍射图案。通过XPS技术分析表面性质和氧化态。Ag 3 PO 4 NPs 对 3,3',5,5'-四甲基联苯胺 (TMB) 具有固有的优异氧化酶模拟特性。当毒死蜱和Ag 3 PO 4 NP纳米酶以适当的方向接近时，毒死蜱被氧化。被氧化的毒死蜱产生硫化物离子和毒死蜱氧分。反应体系中产生的硫化物离子与Ag 3 PO 4相互作用NPs 并通过反馈抑制来抑制其催化活性。实际上，既没有留下任何催化位点来氧化 TMB，也没有出现任何蓝色。因此，这种反馈抑制现象可以感知毒死蜱农药。标准毒死蜱的计算检测限 (LOD) 为~9.97 ppm，并且发现根据K m值计算的 Ag 3 PO 4 NPs 的功效为 0.15 mM。采用标准添加法对从 Pethapur 和 Chiloda 村采集的两个土壤样品进行了实际样品分析。
更新日期：2020-04-01
中文翻译：
通过负反馈抑制磷酸银加氧酶纳米酶的催化活性比色传感毒死蜱
有机磷毒死蜱农药在农业中的大量使用因其对生物的有害影响已成为一个严重的问题。大多数水果、蔬菜和土壤都含有毒死蜱，用水洗不能完全冲洗掉。因此，毒死蜱的选择性和灵敏检测意义重大。在本研究中，探索了 Ag 3 PO 4纳米粒子 (NPs) 的耐人寻味的氧化酶模拟活性，用于快速和选择性地检测毒死蜱农药。与其他昂贵的纳米酶相比， Ag 3 PO 4 NPs 具有催化效率高、稳定性高、单分散性和可重复使用性等优点。一种简单的一步传感。通过FESEM和HR-TEM观察Ag 3 PO 4 NPs的尺寸、形状、晶面和衍射图案。通过XPS技术分析表面性质和氧化态。Ag 3 PO 4 NPs 对 3,3',5,5'-四甲基联苯胺 (TMB) 具有固有的优异氧化酶模拟特性。当毒死蜱和Ag 3 PO 4 NP纳米酶以适当的方向接近时，毒死蜱被氧化。被氧化的毒死蜱产生硫化物离子和毒死蜱氧分。反应体系中产生的硫化物离子与Ag 3 PO 4相互作用NPs 并通过反馈抑制来抑制其催化活性。实际上，既没有留下任何催化位点来氧化 TMB，也没有出现任何蓝色。因此，这种反馈抑制现象可以感知毒死蜱农药。标准毒死蜱的计算检测限 (LOD) 为~9.97 ppm，并且发现根据K m值计算的 Ag 3 PO 4 NPs 的功效为 0.15 mM。采用标准添加法对从 Pethapur 和 Chiloda 村采集的两个土壤样品进行了实际样品分析。
京公网安备 11010802027423号