Changes in gene copy number contribute to genomic instability, the onset and progression of cancer, developmental abnormalities and adaptive potential. The origins of gene amplifications have remained elusive; however, DNA rereplication has been implicated as a source of gene amplifications. The inability to determine which sequences are rereplicated and under what conditions have made it difficult to determine the validity of the proposed models. Here we present Rerep-Seq, a technique that selectively enriches for rereplicated DNA in preparation for analysis by DNA sequencing that can be applied to any species. We validated Rerep-Seq by simulating DNA rereplication in yeast and human cells. Using Rerep-Seq, we demonstrate that rereplication induced in Saccharomyces cerevisiae by deregulated origin licensing is non-random and defined by broad domains that span multiple replication origins and topological boundaries.

中文翻译：

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通过 Rerep-Seq 分离和分析重复复制的 DNA。

基因拷贝数的变化会导致基因组不稳定、癌症的发生和进展、发育异常和适应潜力。基因扩增的起源仍然难以捉摸。然而，DNA 再复制被认为是基因扩增的来源。无法确定哪些序列在什么条件下被重复复制，这使得很难确定所提出模型的有效性。在这里，我们介绍了 Rerep-Seq，这是一种选择性富集重复复制的 DNA 的技术，为 DNA 测序分析做准备，可应用于任何物种。我们通过模拟酵母和人类细胞中的 DNA 再复制来验证 Rerep-Seq。使用 Rerep-Seq，