Angiotensin II induces coordinated calcium bursts in aldosterone-producing adrenal rosettes.,Nature Communications

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Angiotensin II induces coordinated calcium bursts in aldosterone-producing adrenal rosettes.
Nature Communications ( IF 14.7 ) Pub Date : 2020-04-03 , DOI: 10.1038/s41467-020-15408-4
Nick A Guagliardo ₁ , Peter M Klein _{1,

2} , Christina A Gancayco ₃ , Adam Lu _{1,

2} , Sining Leng ₄ , Rany R Makarem ₁ , Chelsea Cho ₁ , Craig G Rusin ₅ , David T Breault ₄ , Paula Q Barrett ₁ , Mark P Beenhakker _{1,

2}

Affiliation

Aldosterone-producing zona glomerulosa (zG) cells of the adrenal gland arrange in distinct multi-cellular rosettes that provide a structural framework for adrenal cortex morphogenesis and plasticity. Whether this cyto-architecture also plays functional roles in signaling remains unexplored. To determine if structure informs function, we generated mice with zG-specific expression of GCaMP3 and imaged zG cells within their native rosette structure. Here we demonstrate that within the rosette, angiotensin II evokes periodic Cav3-dependent calcium events that form bursts that are stereotypic in form. Our data reveal a critical role for angiotensin II in regulating burst occurrence, and a multifunctional role for the rosette structure in activity-prolongation and coordination. Combined our data define the calcium burst as the fundamental unit of zG layer activity evoked by angiotensin II and highlight a novel role for the rosette as a facilitator of cell communication.

中文翻译：

血管紧张素 II 在产生醛固酮的肾上腺玫瑰花结中诱导协调钙爆发。

肾上腺产生醛固酮的球状带 (zG) 细胞排列成不同的多细胞花结，为肾上腺皮质形态发生和可塑性提供结构框架。这种细胞结构是否也在信号传导中发挥功能作用仍有待探索。为了确定结构是否影响功能，我们生成了具有 GCaMP3 的 zG 特异性表达的小鼠，并在其天然花结结构内对 zG 细胞进行了成像。在这里，我们证明，在玫瑰花结内，血管紧张素 II 会诱发周期性的 Cav3 依赖性钙事件，形成形式上固定的爆发。我们的数据揭示了血管紧张素 II 在调节爆发发生中的关键作用，以及玫瑰花结构在活动延长和协调中的多功能作用。结合我们的数据，将钙爆发定义为血管紧张素 II 引起的 zG 层活动的基本单位，并强调玫瑰花结作为细胞通讯促进者的新作用。

更新日期：2020-04-24

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