Paper-based analytical devices (PADs) have encountered a wealth of applications in recent years thanks to the numerous advantages of paper as a support. A silver nanoflower (AgNF) modified paper-based dual substrate for both surface-enhanced Raman spectroscopy (SERS) and ambient pressure paper spray mass spectrometry (PS-MS) was developed. AgNFs were immobilized on nylon-coated paper modified with silver and ethylenediamine. The developed substrate was characterized via scanning electron microscopy and infrared spectroscopy. The densely packed nanoscale petals of the AgNFs lead to a large number of so-called hot spots at their overlapping points, which result in an enhancement of the Raman signal. In addition, the presence of the AgNFs produces an increase in the sensitivity of the mass spectrometric analysis as compared with bare paper and nylon/Ag-coated paper. The dual substrate was evaluated for the identification and quantification of ketoprofen in aqueous standards as well as human saliva from healthy volunteers. The method enables the determination of ketoprofen with a limit of detection and limit of quantification via PS-MS of 0.023 and 0.076 mg L-1, respectively, with a relative standard deviation (RSD) of 3.4% at a concentration of 0.1 mg L-1. This dual substrate enables the simple and fast detection of ketoprofen with minimal sample preparation, providing complementary Raman and mass spectrometric information. Graphical abstract.

中文翻译：

---

银纳米花涂层纸作为表面增强拉曼光谱和环境压力质谱分析的双重基材。

由于纸张作为支撑物的众多优势，纸质分析设备（PAD）近年来得到了广泛的应用。开发了一种用于表面增强拉曼光谱 (SERS) 和环境压力纸喷雾质谱 (PS-MS) 的银纳米花 (AgNF) 改性纸基双基底。AgNFs 固定在用银和乙二胺改性的尼龙涂层纸上。通过扫描电子显微镜和红外光谱对开发的基板进行表征。AgNFs 的密集纳米级花瓣在它们的重叠点处导致大量所谓的热点，从而导致拉曼信号的增强。此外，与裸纸和尼龙/Ag 涂层纸相比，AgNFs 的存在提高了质谱分析的灵敏度。评估了双底物对水性标准中酮洛芬以及健康志愿者人类唾液中酮洛芬的鉴定和定量。该方法能够测定酮洛芬，通过 PS-MS 的检测限和定量限分别为 0.023 和 0.076 mg L-1，相对标准偏差 (RSD) 为 3.4%，浓度为 0.1 mg L- 1. 这种双底物能够以最少的样品制备简单快速地检测酮洛芬，提供互补的拉曼和质谱信息。图形概要。评估了双底物对水性标准中酮洛芬以及健康志愿者人类唾液中酮洛芬的鉴定和定量。该方法能够测定酮洛芬，通过 PS-MS 的检测限和定量限分别为 0.023 和 0.076 mg L-1，相对标准偏差 (RSD) 为 3.4%，浓度为 0.1 mg L- 1. 这种双底物能够以最少的样品制备简单快速地检测酮洛芬，提供互补的拉曼和质谱信息。图形概要。评估了双底物对水性标准中酮洛芬以及健康志愿者人类唾液中酮洛芬的鉴定和定量。该方法能够测定酮洛芬，通过 PS-MS 的检测限和定量限分别为 0.023 和 0.076 mg L-1，相对标准偏差 (RSD) 为 3.4%，浓度为 0.1 mg L- 1. 这种双底物能够以最少的样品制备简单快速地检测酮洛芬，提供互补的拉曼和质谱信息。图形概要。4%，浓度为 0.1 mg L-1。这种双底物能够以最少的样品制备简单快速地检测酮洛芬，提供互补的拉曼和质谱信息。图形概要。4%，浓度为 0.1 mg L-1。这种双底物能够以最少的样品制备简单快速地检测酮洛芬，提供互补的拉曼和质谱信息。图形概要。