Monitoring of hydrogen peroxide (H2O2) in living cells has high significance for understanding its functions. We herein report an enzymeless H2O2 sensor consisting of a previously activated screen-printed carbon electrode modified with Pt nanoparticles electrogenerated on a supporting conductive layer of polyazure A-dodecyl sulfate. This electrode was used to investigate the dynamic process of H2O2 release from living grapevine cells under different (a)biotic stresses. The modified surfaces were characterized by FESEM/EDX, EIS and cyclic voltammetry. Sensor analytical performance was studied in a cell culture medium under aerobic conditions, as required for cell survival. In relation to the synergistic effect between the metal nanoparticles and the conjugated polymer, this electrode showed good stability, excellent analytical performance combined with a rapid response (<2s) and limit of detection of 24.9 nM in the culture medium. The modified electrodes could fulfill the real-time measurement requirement of H2O2 release from living plant cells to the extracellular medium operating continuously, even in experiments lasting more than 12 h. Methyl jasmonate, L-methionine, clopyralid and the fungus Botrytis cinerea were the eliciting agents chosen to induce oxidative stress in the plant cells. This work demonstrates the huge potential of this sensor for the real-time tracking of the H2O2 released from living cells under different physiological conditions.

中文翻译：

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基于PtNPs @ polyazure A的非酶丝网印刷传感器，用于实时跟踪从活植物细胞分泌的H2O2。

监测活细胞中的过氧化氢（H2O2）对于了解其功能具有很高的意义。我们在此报告了一种无酶的H2O2传感器，该传感器由先前活化的丝网印刷碳电极组成，该电极经Pt纳米粒子修饰，该电极在聚天青A-十二烷基硫酸盐的支撑导电层上电生成。该电极用于研究在不同的（a）生物胁迫下活葡萄细胞释放H2O2的动态过程。通过FESEM / EDX，EIS和循环伏安法对改性后的表面进行表征。根据细胞存活的需要，在有氧条件下的细胞培养基中研究了传感器的分析性能。关于金属纳米粒子与共轭聚合物之间的协同效应，该电极显示出良好的稳定性，出色的分析性能与快速响应（<2s）和培养基中24.9 nM的检测限相结合。修饰的电极可以满足从活的植物细胞到连续运行的细胞外介质中H2O2释放的实时测量要求，即使在持续超过12小时的实验中也是如此。茉莉酸甲酯，L-甲硫氨酸，氯吡格雷和灰葡萄孢菌是被选为在植物细胞中诱导氧化应激的引发剂。这项工作证明了这种传感器在不同生理条件下实时跟踪从活细胞释放的H2O2的巨大潜力。修饰的电极可以满足从活的植物细胞到连续运行的细胞外介质中H2O2释放的实时测量要求，即使在持续超过12小时的实验中也是如此。茉莉酸甲酯，L-甲硫氨酸，氯吡格雷和灰葡萄孢菌是被选为在植物细胞中诱导氧化应激的引发剂。这项工作证明了这种传感器在不同生理条件下实时跟踪从活细胞释放的H2O2的巨大潜力。修饰的电极可以满足从活体植物细胞到连续运行的细胞外介质中H2O2释放的实时测量要求，即使在持续超过12小时的实验中也是如此。茉莉酸甲酯，L-甲硫氨酸，氯吡格雷和灰葡萄孢菌是被选为在植物细胞中诱导氧化应激的引发剂。这项工作证明了这种传感器在不同生理条件下实时跟踪从活细胞释放的H2O2的巨大潜力。