Certain types of oligosaccharides are especially interesting due to their high commercial value, however, the traditional oligosaccharide fabrication process is neither sustainable nor tailorable for obtainment of commercial standard oligosaccharide streams. The use of enzymatic membrane reactors (EMR) is proposed as a sustainable strategy to obtain oligosaccharides through a coupled bioreaction-separation process. In such process, glycosidic enzymes function as catalysts and membranes as selective barriers tailoring the molecular weight of products.

Most of previous studies on oligosaccharides production by EMR have focused either on optimal operation of the enzyme reaction or the membrane filtration separately, but none of them have considered the implications of coupling both steps together. It is therefore the aim of this review to critically assess the available literature on the topic from a novel point of view: by paying special attention to the interactions between enzyme reaction-membrane filtration, which are critical for the performance of the overall process. Depending on the reaction conditions, the generated fouling directly affecting filtration performance varies. Such fouling depends on the membrane type and operation mode, which will in turn have an influence on the enzyme performance. The proposed review aims at providing a critical overview of the variables influencing performance and will provide tips for optimal configuration and operation of the coupled system.

某些类型的寡糖由于其高商业价值而特别引起人们的兴趣，但是，传统的寡糖制造工艺既不可持续，也不适合获得商业标准寡糖物流。提议使用酶促膜反应器（EMR）作为通过偶联生物反应分离过程获得寡糖的可持续策略。在这样的过程中，糖苷酶起着催化剂的作用，而膜则起着调节产品分子量的选择性屏障的作用。

以前有关EMR生产寡糖的大多数研究都集中在酶反应的最佳操作或膜过滤上，但都没有考虑将两个步骤结合在一起的含义。因此，本综述的目的是从新颖的角度批判性地评估有关该主题的可用文献：通过特别注意酶反应-膜过滤之间的相互作用，这对于整个过程的执行至关重要。根据反应条件，直接影响过滤性能的结垢情况会有所不同。这种结垢取决于膜的类型和操作模式，这将反过来影响酶的性能。