Spermine, a biomarker of cancerous cells, is electrochemically detected using poly(β-cyclodextrin) recognition element electro-polymerized on a Cu-carbon nanofiber (CNF)-dispersed carbon film. The carbon film was prepared by carbonization of a phenol-formaldehyde polymeric film in situ dispersed with Cu nanoparticles during the polymerization stage, and the CNFs were grown on the carbon film using chemical vapor deposition. The prepared biosensor showed good repeatability and reproducibility in the clinical spermine samples when tested using differential pulse voltammetry over the concentration range 0.005–100 mg L^-1. The limit of detection was considerably low at 0.001 mg L^-1. Besides showing good linearity with R² = 0.9885 and 0.9955 over the concentration ranges 0.005–2 and 2–100 mg L^-1, respectively, the measurements were unaffected by interfering biomolecules such as cholesterol, glutamine, and putrescine in blood. The technique including materials and methods described in this study can be modified to develop biosensors for the fast and easy detection of the biomolecules that can serve as biomarkers of the other chronic diseases.

精胺是癌细胞的生物标志物，是使用电聚合在分散有铜碳纳米纤维（CNF）的碳膜上的聚（β-环糊精）识别元件进行电化学检测的。通过在聚合阶段碳化与铜纳米颗粒原位分散的苯酚-甲醛聚合物膜来制备碳膜，并使用化学气相沉积法在碳膜上生长CNF。当在0.005–100 mg L ^-1的浓度范围内使用差分脉冲伏安法测试时，所制备的生物传感器在临床精胺样品中显示出良好的可重复性和重现性。检出限在0.001 mg L ^-1时相当低。除了与R ²表现出良好的线性关系分别在0.005–2和2–100 mg L ^-1的浓度范围内分别为0.9885和0.9955 ，测量不受血液中胆固醇，谷氨酰胺和腐胺等干扰生物分子的影响。可以修改本研究中描述的包括材料和方法在内的技术，以开发生物传感器，以快速，轻松地检测可以用作其他慢性疾病生物标志物的生物分子。