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Physiological quantification and molecular detection (BeNRT1.1) of neosinocalamus (bambusa emeiensis) treated with different types of nitrogen fertilizers
Industrial Crops and Products ( IF 5.6 ) Pub Date : 2020-04-02 , DOI: 10.1016/j.indcrop.2020.112323
Zhijian Long , Muhammad Imran , Shanglian Hu , Xuegang Luo , Ying Cao , Naseem Samo , Daoyong Gong

Nitrogen based fertilizers have great potential for the ontogenesis and improvement of plants on sustainable basis. This study aims to unveil the effects of nitrogen based fertilizers on physiology, biochemistry and photochemistry of Bambusa emeiensis L. C. Chia & H. L. Fung; along with cloning and expression of a nitrate transporter gene from NRT1 family (BeNRT1.1). Our data revealed that NO3N has a very positive effect on the basal diameter of clumping. The plants treated with NO3N demonstrated a decrease in the fiber length along with the cellulose content; while the increase has been observed in the lignin content and nitrate reductase activity. Furthermore, the fertilizers NO3N and (−CONH2) are observed to trigger the soluble protein content in the base of the shoot. While the plants treated with NH4+-N shown morphological effects on the thickness of the inner fiber strand and the height of central vascular bundles. It was found that the parenchyma cells between the two adjacent vascular bundles were significantly thicker than that of the control group. The NH4+-N also increased the sugar content at the base of the shoots and increased the glutamate synthase activity. The different kinds of nitrogen fertilizers also have positive effects on chlorophyll content. Moreover, the NRT gene was cloned and characterized; which demonstrated that the gene BeNRT1.1 (Accession No. KY421770), have an open reading frame (ORF) of 1611 bp, encoding a total of 536 amino acid residues. Phylogenetic analysis showed that the amino acid residues of BeNRT1.1 were similar to that of AtNRT1.1 genes in Arabidopsis thaliana which is known for nitrate transport. qRT-PCR data revealed that BeNRT1.1 was highly expressed in the base of the shoots when treated with NO3N fertilizer.



中文翻译:

生理量化和分子检测(BeNRT 1.1慈的(竹峨眉)与不同类型的氮肥处理

氮基肥料在可持续基础上对植物的发生和改良具有巨大潜力。本研究旨在揭示氮基肥料对峨眉竹的生理,生化和光化学的影响以及来自NRT1家族(BeNRT 1.1)的硝酸盐转运蛋白基因的克隆和表达。我们的数据表明,NO 3 - N对团块的基径有非常积极的影响。由于没有处理的植物3 -N表明纤维长度和纤维素含量一起减少;同时观察到木质素含量和硝酸还原酶活性的增加。此外,观察到肥料NO 3 - N和(-CONH 2)触发了枝条基部的可溶性蛋白质含量。NH 4 + -N处理的植物对内部纤维束的厚度和中央血管束的高度显示出形态学影响。发现两个相邻血管束之间的薄壁组织细胞明显厚于对照组。NH 4 +-N还增加了芽基部的糖含量并增加了谷氨酸合酶活性。不同种类的氮肥对叶绿素含量也有积极作用。此外,克隆并鉴定了NRT基因。证明基因BeNRT 1.1(登录号KY421770)具有1611 bp的开放阅读框(ORF),编码总共536个氨基酸残基。系统进化分析表明,BeNRT 1.1的氨基酸残基与拟南芥中硝酸盐转运的AtNRT 1.1基因相似。qRT-PCR数据显示BeNRT 1.1当用NO 3 - N肥料处理时,其在芽的基部高度表达。

更新日期:2020-04-03
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