In this work we analyzed the effects of Sulfosato Touchdown®, a glyphosate-based herbicide, on the ontogenic development and biochemical markers of the freshwater copepod Notodiaptomus carteri. A 30-days life-cycle experiment was carried out with three different glyphosate concentrations (0, 0.38, and 0.81 mg L⁻¹) to analyze the developmental time from nauplii to adult copepods and their individual growth. An additional 10-days experiment with the same glyphosate concentrations was designed to evaluate the energy reserves (glycogen, proteins and lipids) and the activity of three antioxidant enzymes, superoxide dismutase (SOD), catalase, and glutathione-S-transferase (GST) in adult copepods, separately for females and males. We found that the lowest glyphosate concentration increased the nauplii and total development time. The highest glyphosate concentration prevented copepods from reaching the adult stage, inhibited the growth of the first copepodite stage and increased the GST and SOD activity in adult females. According to our results, the presence of this herbicide in freshwater systems could impose a risk in the ecological role of copepods in nature. This study will contribute to propose the Notodiaptomus genus as model specie for monitoring purposes in the Neotropical aquatic systems.

在这项工作中，我们分析了 Sulfosato Touchdown®（一种草甘膦除草剂）对淡水桡足类Notdiaptomus carteri个体发育和生化标记的影响。采用三种不同浓度（0、0.38和0.81 mg L ^-1 ）草甘膦进行30天生命周期实验，分析从无节幼体到成年桡足类的发育时间及其个体生长情况。另一项为期 10 天的实验采用相同的草甘膦浓度，旨在评估能量储备（糖原、蛋白质和脂质）以及三种抗氧化酶（超氧化物歧化酶 (SOD)、过氧化氢酶和谷胱甘肽-S-转移酶 (GST)）的活性在成年桡足类动物中，雌性和雄性分别。我们发现最低的草甘膦浓度增加了无节幼体和总发育时间。最高草甘膦浓度阻止桡足类达到成年阶段，抑制第一个桡足类阶段的生长并增加成年雌性的GST和SOD活性。根据我们的结果，淡水系统中这种除草剂的存在可能会对桡足类在自然界中的生态作用造成风险。这项研究将有助于提议将Notdiaptomus属作为新热带水生系统监测目的的模式物种。