Differentiation of soluble aqueous humor metabolites in primary open angle glaucoma and controls.,Experimental Eye Research

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Differentiation of soluble aqueous humor metabolites in primary open angle glaucoma and controls.
Experimental Eye Research ( IF 3.0 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.exer.2020.108024
Ciara Myer ₁ , Jordan Perez ₂ , Leila Abdelrahman ₁ , Roberto Mendez ₃ , Ram B Khattri ₄ , Anna K Junk ₅ , Sanjoy K Bhattacharya ₁

Affiliation

We report an analysis of the aqueous humor (AH) metabolome of primary open angle glaucoma (POAG) in comparison to normal controls. The AH samples were obtained from human donors [control (n = 35), POAG (n = 23)]. The AH samples were subjected to one-dimensional 1H nuclear magnetic resonance (NMR) analyses on a Bruker Avance 600 MHz instrument with a 1.7 mM NMR probe. The same samples were then subjected to isotopic ratio outlier analysis (IROA) using a Q Exactive orbitrap mass spectrometer after chromatography on an Accela 600 HPLC. Clusterfinder Build 3.1.10 was used for identification and quantification based on long-term metabolite matrix standards. In total, 278 metabolites were identified in control samples and 273 in POAG AH. The metabolites identified were fed into previously reported proteome and genome information and the OmicsNet interaction network generator to construct a protein-metabolite interactions network with an embedded protein-protein network. Significant differences in metabolite composition in POAG compared to controls were identified indicating potential protein/gene pathways associated with these metabolites. These results will expand our previous understanding of the impeded AH metabolite composition, provide new insight into the regulation of AH outflow, and likely aid in future AH and trabecular meshwork multi-omics network analyses.

中文翻译：

原发性开角型青光眼和对照中可溶性房水代谢产物的分化。

我们报告原发性开角型青光眼（POAG）与正常对照相比房水（AH）代谢组的分析。AH样本是从人类供体中获得的[对照组（n = 35），POAG（n = 23）]。在带有1.7 mM NMR探针的Bruker Avance 600 MHz仪器上对AH样品进行一维1H核磁共振（NMR）分析。然后，在Accela 600 HPLC上进行色谱分离后，使用Q Exactive Orbitrap质谱仪对相同样品进行同位素比率离群值分析（IROA）。Clusterfinder Build 3.1.10用于基于长期代谢产物基质标准品的鉴定和定量。总共在对照样品中鉴定出278种代谢产物，在POAG AH中鉴定出273种代谢产物。鉴定出的代谢物被送入先前报道的蛋白质组和基因组信息，以及OmicsNet相互作用网络生成器，以构建具有嵌入式蛋白质-蛋白质网络的蛋白质-代谢物相互作用网络。鉴定出与对照相比，POAG中代谢物组成的显着差异，表明与这些代谢物相关的潜在蛋白质/基因途径。这些结果将扩大我们先前对受阻的AH代谢物组成的了解，为AH流出的调节提供新的见解，并可能有助于将来的AH和小梁网多组学网络分析。鉴定出与对照相比，POAG中代谢物组成的显着差异，表明与这些代谢物相关的潜在蛋白质/基因途径。这些结果将扩大我们先前对受阻的AH代谢物组成的了解，为AH流出的调节提供新的见解，并可能有助于将来的AH和小梁网多组学网络分析。鉴定出与对照相比，POAG中代谢物组成的显着差异，表明与这些代谢物相关的潜在蛋白质/基因途径。这些结果将扩大我们先前对受阻的AH代谢物组成的了解，为AH流出的调节提供新的见解，并可能有助于将来的AH和小梁网多组学网络分析。

更新日期：2020-04-03

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