The effect of high pressure (HP) and pulsed electric field (PEF) treatments combined or not with heat on denaturation and allergenicity of Pru p 3, the major allergenic protein of peach, was studied. Denaturation of Pru p 3, determined by ELISA using rabbit IgG, occurred when the protein was treated at 500 and 600 MPa at 20 °C and at 400 MPa at 50 °C. PEF treatment at 25 kV/cm at 50 °C denatures Pru p 3. Allergenicity of Pru p 3 was estimated in vitro by a competitive fluorescent immunoassay using three pools of sera from peach allergic patients. Any treatment applied did not show to influence the binding of Pru p 3 to IgE. When HP and PEF treated samples were tested by the prick test, the skin response was dependent on the particular sensitization of each patient, obtaining an increased reaction in more than 50% of individuals.

中文翻译：

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高压和脉冲电场对桃Pru p 3蛋白变性和变应原性的影响。

研究了高压（HP）和脉冲电场（PEF）处理与不加热相结合对桃的主要致敏蛋白Pru p 3的变性和致敏性的影响。当蛋白在20℃下分别在500和600 MPa下以及50℃下在400 MPa下处理时，通过ELISA使用兔IgG测定的Pru p 3发生变性。在50℃下以25 kV / cm的PEF处理使Pru p 3变性。通过竞争荧光免疫测定法，使用来自桃子变态反应患者的三份血清在体外评估了Pru p 3的致敏性。所应用的任何处理均未显示影响Pru p 3与IgE的结合。当通过针刺试验对经过HP和PEF处理的样品进行测试时，皮肤反应取决于每位患者的特殊敏感性，超过50％的患者反应增强。