Heparin (Hep) is a mucopolysaccharide sulfate anticoagulant drug widely used in clinic and thus the quantification of Hep concentration is of great significance. In this work, a simple resonance light scattering (RLS) method for the determination of Hep concentration using plasmonic copper selenide nanoparticles (Cu_2-xSe NPs) was developed. The positively-charged Cu_2-xSe NPs capped by cetyltrimethyl ammonium bromide (CTAB) were aggregated by negatively-charged Hep, consequently inducing a significant enhancement in the RLS signals of plasmonic Cu_2-xSe NPs. Under the optimal reaction conditions, the increased RLS intensity is linearly correlated with the concentrations of Hep in the range of 0.01–0.60 μg mL⁻¹ (R² = 0.999), with a low detection limit (LOD) of 4.0 ng mL⁻¹ (3σ). This method offered a simple, sensitive and selective strategy for determining Hep, which can be further successfully applied to quantify Hep in the heparin sodium injection.

肝素（Hep）是临床上广泛使用的粘多糖硫酸盐抗凝药，因此对Hep浓度的定量具有重要意义。在这项工作中，开发了一种简单的共振光散射（RLS）方法，使用等离激元硒化铜纳米颗粒（Cu _2-x Se NPs）测定Hep浓度。被十六烷基三甲基溴化铵（CTAB）封端的带正电的Cu _2-x Se NPs被带负电的Hep聚集，因此导致了等离子Cu _2-x Se NPs的RLS信号显着增强。在最佳反应条件下，增加的RLS强度与Hep的浓度在0.01–0.60μgmL ^-1  （R ² = 0.999），检测限（LOD）较低，为4.0 ng mL ^-1（3σ）。该方法提供了一种简单，灵敏和选择性的测定Hep的策略，可将其进一步成功地用于定量肝素钠注射液中的Hep。