当前位置: X-MOL 学术Colloids Surf. B Biointerfaces › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Anti-microbial and anti-biofilm activities of combined chelerythrine-sanguinarine and mode of action against Candida albicans and Cryptococcus neoformans in vitro.
Colloids and Surfaces B: Biointerfaces ( IF 5.4 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.colsurfb.2020.111003
Weidong Qian 1 , Min Yang 2 , Xinchen Li 2 , Zhaohuan Sun 2 , Yongdong Li 3 , Xuejun Wang 4 , Ting Wang 2
Affiliation  

The increasing prevalence of fungal infections coupled with emerging drug resistance has stimulated an urgent need to explore new and effective antifungal agents. Sanguinarine and chelerythrine constitute alkaloids that have exhibited antifungal activities. However, the effects of a 1:1 mixture of these agents against Candida albicans and Cryptococcus neoformans have remained largely unexplored. The purpose of this study was to assess the anti-fungal and anti-biofilm efficacy of combined chelerythrine-sanguinarine against C. albicans and C. neoformans in vitro. Combined chelerythrine-sanguinarine inhibited C. albicans and C. neoformans growth with minimum inhibitory concentrations (MICs) of 2 and 16 μg/mL, respectively, and effectively inhibited adhesion and biofilm formation of these pathogens at minimum biofilm inhibitory concentrations of 1 and 8 μg/mL. Notably, the mixture significantly eradicated mature C. albicans and C. neoformans biofilms at 8 and 128 μg/mL, respectively. In particular, the mixture was found to disrupt cell membrane integrity and enhance penetration of antibiotics into fungal cells, suggesting its antifungal mode of action. Hence, combined chelerythrine-sanguinarine shows promise as a potential anti-fungal and anti-biofilm agent for the management of serious infections caused by C. albicans and C. neoformans.

中文翻译:

结合白屈菜红碱-血红素碱的抗微生物和生物膜活性以及体外对白色念珠菌和新隐球菌的作用方式。

真菌感染的流行与新出现的耐药性激增,迫切需要探索新的有效的抗真菌剂。血红素碱和白屈菜红碱构成具有抗真菌活性的生物碱。但是,这些试剂对白念珠菌和新隐球菌的1:1混合物的作用在很大程度上尚待探索。这项研究的目的是评估结合白屈菜红碱-血红素碱在体外对白色念珠菌和新形成梭菌的抗真菌和生物膜功效。结合白屈菜红碱-血红素碱可抑制白色念珠菌和新孢子虫的生长,最小抑菌浓度(MIC)分别为2和16μg/ mL,并在1和8μg/ mL的最小生物膜抑制浓度下有效抑制这些病原体的粘附和生物膜形成。值得注意的是,该混合物分别以8和128μg/ mL的浓度显着消除了白色念珠菌和新形成梭菌的生物膜。特别地,发现该混合物破坏细胞膜完整性并增强抗生素向真菌细胞的渗透,表明其抗真菌作用方式。因此,白屈菜红碱-血红碱联合用药显示出有望作为一种潜在的抗真菌和抗生物膜剂,用于处理由白色念珠菌和新孢子虫引起的严重感染。发现该混合物破坏细胞膜完整性并增强抗生素向真菌细胞的渗透,表明其抗真菌作用模式。因此,白屈菜红碱-血红碱联合用药显示出有望作为一种潜在的抗真菌和抗生物膜剂,用于处理由白色念珠菌和新孢子虫引起的严重感染。发现该混合物破坏细胞膜完整性并增强抗生素向真菌细胞的渗透,表明其抗真菌作用模式。因此,白屈菜红碱-血红碱联合用药显示出有望作为一种潜在的抗真菌和抗生物膜剂,用于处理由白色念珠菌和新孢子虫引起的严重感染。
更新日期:2020-04-03
down
wechat
bug