Water in oil emulsions have turned into interesting and attractive resources for enhanced oil recovery (EOR). The stability of emulsions is important for its efficiency as EOR agents. The objective of this study was to investigate the simultaneous influence of ultrasound waves and microorganisms on the stability of water-in-oil emulsions. To this end, using an Iranian crude oil, two types of waters, surfactants and three types of biosurfactants producing microorganisms the impacts of temperature, ultrasonic frequency, and radiation time on the stability, viscosity and surface tension of water-in-oil emulsions were studied. From the results of this study, it can be pointed out that compared to the conventional surface-active agents; biosurfactants have a higher impact on the stability of emulsions. In addition, under the optimum conditions of this study, the surface tension decreased about 15% and the stability increased nearby 20%. Ultrasonic radiation reduces emulsion stability by about 6%. The T.F. species have the greatest effect on the emulsion stability up to 70 °C (more than 85%). Micromodel flooding experiments showed that injection of T.F biosurfactants emulsion provides the highest oil recovery due to their high stability, high viscosity, and low surface tension.

油包水乳状液已成为增强油采收率（EOR）的有趣且有吸引力的资源。乳液的稳定性对其作为EOR剂的效率至关重要。这项研究的目的是研究超声波和微生物对油包水乳液稳定性的同时影响。为此，使用伊朗原油，两种类型的水，表面活性剂和三种类型的产生微生物的生物表面活性剂，温度，超声频率和辐射时间对油包水型乳液的稳定性，粘度和表面张力的影响如下：研究。从这项研究的结果，可以指出，与传统的表面活性剂相比；生物表面活性剂对乳液的稳定性有较高的影响。此外，在这项研究的最佳条件下，表面张力降低了约15％，稳定性提高了约20％。超声波辐射会使乳液稳定性降低约6％。TF种类对高达70°C（大于85％）的乳液稳定性影响最大。微观模型驱油实验表明，由于TF生物表面活性剂乳液的高稳定性，高粘度和低表面张力，因此可提供最高的采油率。