MpFEW RHIZOIDS1 miRNA-Mediated Lateral Inhibition Controls Rhizoid Cell Patterning in Marchantia polymorpha.,Current Biology

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MpFEW RHIZOIDS1 miRNA-Mediated Lateral Inhibition Controls Rhizoid Cell Patterning in Marchantia polymorpha.
Current Biology ( IF 8.1 ) Pub Date : 2020-04-02 , DOI: 10.1016/j.cub.2020.03.032
Anna Thamm ₁ , Timothy E Saunders ₂ , Liam Dolan ₁

Affiliation

Lateral inhibition patterns differentiated cell types among equivalent cells during development in bacteria, metazoans, and plants. Tip-growing rhizoid cells develop among flat epidermal cells in the epidermis of the early-diverging land plant Marchantia polymorpha. We show that the majority of rhizoid cells develop individually, but some develop in linear, one-dimensional groups (chains) of between 2 and 7 rhizoid cells in wild-type plants. The distribution of rhizoid cells can be accounted for within a simple cellular automata model of lateral inhibition. The model predicted that in the absence of lateral inhibition, two-dimensional rhizoid cell groups (clusters) form. These can be larger than those formed with lateral inhibition. M. polymorpha rhizoid differentiation is positively regulated by the ROOT HAIR DEFECTIVE SIX-LIKE1 (MpRSL1) basic-helix-loop-helix (bHLH) transcription factor, which is directly repressed by the FEW RHIZOIDS1 (MpFRH1) microRNA (miRNA). To test if MpFRH1 miRNA acts during lateral inhibition, we generated loss-of-function (lof) mutants without the MpFRH1 miRNA. Two-dimensional clusters of rhizoids develop in Mpfrh1lof mutants as predicted by the model for plants that lack lateral inhibition. Furthermore, two-dimensional clusters of up to 9 rhizoid cells developed in the Mpfrh1lof mutants compared to a maximum number of 7 observed in wild-type groups. The higher steady-state levels of MpRSL1 mRNA in Mpfrh1lof mutants indicate that MpFRH1-mediated lateral inhibition involves the repression of MpRSL1 activity. Together, the modeling and genetic data indicate that MpFRH1 miRNA mediates lateral inhibition by repressing MpRSL1 during pattern formation in the M. polymorpha epidermis.

中文翻译：

MpFEW RHIZOIDS1 miRNA 介导的横向抑制控制 Marchantia polymorpha 中的根状细胞模式。

在细菌、后生动物和植物的发育过程中，横向抑制模式区分了等效细胞中的细胞类型。尖端生长的根状细胞在早期发散的陆生植物马钱蒂亚的表皮中的扁平表皮细胞中发育。我们表明大多数根状细胞单独发育，但有些在野生型植物中以 2 到 7 个根状细胞的线性、一维组（链）形式发育。根状细胞的分布可以在一个简单的侧抑制细胞自动机模型中解释。该模型预测，在没有侧向抑制的情况下，二维根状细胞群（簇）形成。这些可能大于侧抑制形成的那些。M。地黄根状茎分化受 ROOT HAIR DEFECTIVE SIX-LIKE1 (MpRSL1) 碱性-螺旋-环-螺旋 (bHLH) 转录因子正调控，该转录因子被 FEW RHIZOIDS1 (MpFRH1) microRNA (miRNA) 直接抑制。为了测试 MpFRH1 miRNA 是否在侧向抑制期间起作用，我们生成了没有 MpFRH1 miRNA 的功能丧失 (lof) 突变体。正如模型预测的那样，缺乏侧向抑制的植物会在 Mpfrh1lof 突变体中形成二维的根状茎簇。此外，与野生型组中观察到的最大数量 7 相比，在 Mpfrh1lof 突变体中形成了多达 9 个根状细胞的二维簇。Mpfrh1lof 突变体中 MpRSL1 mRNA 的较高稳态水平表明 MpFRH1 介导的侧向抑制涉及 MpRSL1 活性的抑制。一起，

更新日期：2020-04-02

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