Mammalian cells are the main tool for the production of therapeutic proteins, viruses for gene therapy, and cells for cell therapy. In production processes cell metabolism is the main driver that causes changes in the growth environment and affects productivity and product quality. Of all nutrients, glucose has the most prominent impact on bioprocesses. We summarize recent findings on the regulation of glucose and energy metabolism in cultured cells. Local allosteric regulations and post-translational modifications of enzymes in metabolic networks interplay with global signaling and transcriptional regulation. These regulatory networks sustain homeostasis across the cytosolic and mitochondrial compartments. Understanding the regulation of glucose metabolism and metabolic state is crucial for enhancing process productivity and product quality.

哺乳动物细胞是生产治疗性蛋白质、用于基因治疗的病毒和用于细胞治疗的细胞的主要工具。在生产过程中，细胞代谢是导致生长环境变化并影响生产力和产品质量的主要驱动因素。在所有营养素中，葡萄糖对生物过程的影响最为突出。我们总结了关于培养细胞中葡萄糖和能量代谢调节的最新发现。代谢网络中酶的局部变构调节和翻译后修饰与全局信号和转录调节相互作用。这些调节网络维持细胞溶质和线粒体区室的稳态。了解葡萄糖代谢和代谢状态的调节对于提高过程生产力和产品质量至关重要。