We present TADsplimer, the first computational tool to systematically detect topologically associating domain (TAD) splits and mergers across the genome between Hi-C samples. TADsplimer recaptures splits and mergers of TADs with high accuracy in simulation analyses and defines hundreds of TAD splits and mergers between pairs of different cell types, such as endothelial cells and fibroblasts. Our work reveals a key role for TAD remodeling in epigenetic regulation of transcription and delivers the first tool for the community to perform dynamic analysis of TAD splits and mergers in numerous biological and disease models.

中文翻译：

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TADsplimer 揭示了用于转录表观遗传调控的拓扑相关域的分裂和合并

我们提出了 TADsplimer，这是第一个系统地检测 Hi-C 样本之间跨基因组的拓扑关联域 (TAD) 分裂和合并的计算工具。TADsplimer 在模拟分析中以高精度重新捕获 TAD 的分裂和合并，并定义了成对不同细胞类型（例如内皮细胞和成纤维细胞）之间的数百个 TAD 分裂和合并。我们的工作揭示了 TAD 重塑在转录表观遗传调控中的关键作用，并为社区提供了第一个工具，用于在众多生物和疾病模型中对 TAD 分裂和合并进行动态分析。