Cyclic Peptides in Seed of Annona muricata Are Ribosomally Synthesized.,Journal of Natural Products

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Cyclic Peptides in Seed of Annona muricata Are Ribosomally Synthesized.
Journal of Natural Products ( IF 3.3 ) Pub Date : 2020-04-02 , DOI: 10.1021/acs.jnatprod.9b01209
Mark F Fisher ₁ , Jingjing Zhang ₁ , Oliver Berkowitz ₂ , James Whelan ₂ , Joshua S Mylne ₁

Affiliation

Small, cyclic peptides are reported to have many bioactivities. In bacteria and fungi, they can be made by nonribosomal peptide synthetases, but in plants they are exclusively ribosomal. Cyclic peptides from the Annona genus possess cytotoxic and anti-inflammatory activities, but their biosynthesis is unknown. The medicinal soursop plant, Annona muricata, contains annomuricatins A (cyclo-PGFVSA) and B (cyclo-PNAWLGT). Here, using de novo transcriptomics and tandem mass spectrometry, we identify a suite of short transcripts for precursor proteins for 10 validated annomuricatins, 9 of which are novel. In their precursors, annomuricatins are preceded by an absolutely conserved Glu and each peptide sequence has a conserved proto-C-terminal Pro, revealing parallels with the segetalin orbitides from the seed of Vaccaria hispanica, which are processed through ligation by a prolyl oligopeptidase in a transpeptidation reaction.

中文翻译：

核糖体Annona muricata种子中的环状肽是合成的。

据报道，小的环状肽具有许多生物活性。在细菌和真菌中，它们可由非核糖体肽合成酶制得，但在植物中，它们仅是核糖体。Annona属的环肽具有细胞毒性和抗炎活性，但其生物合成尚不清楚。药用刺五加植物番荔枝番荔枝（Annona muricata）含有anuriuricatins A（环-PGFVSA）和B（环-PNAWLGT）。在这里，我们使用从头转录组学和串联质谱技术，为10个经过验证的ananuricatins（其中9个是新颖的）识别了一组前体蛋白的短转录本。在其前体中，无尿尿素素的前面是绝对保守的Glu，每个肽序列都有一个保守的C端Pro，与西班牙痘苗种子中的segetalin orbitides相似，

更新日期：2020-04-02

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