Bioactive ECM Mimic Hyaluronic Acid Dressing via Sustained Releasing of bFGF for Enhancing Skin Wound Healing,ACS Applied Bio Materials

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Bioactive ECM Mimic Hyaluronic Acid Dressing via Sustained Releasing of bFGF for Enhancing Skin Wound Healing
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-04-02 , DOI: 10.1021/acsabm.0c00096
Anqi Chen ₁ , Wen Huang ₁ , Liang Wu _{1,

2} , Ying An ₁ , Tengxiao Xuan ₁ , Huacheng He ₃ , Mengqi Ye ₃ , Lamei Qi ₂ , Jiang Wu ₁

Affiliation

Successful dermal wound regeneration requires the coordination of repair cells and cellular signals with the extracellular matrix (ECM), which serves as an indispensable mechanical and biological supporter for cell functions and communications with varied cytokines during healing processes. Here, we developed an injectable bioactive wound dressing, methacrylated hyaluronic acid (Me-HA)-based hydrogel loading with basic fibroblast growth factor (bFGF), endowing the dressing with the pleiotropic bioactivity to mimic natural ECM. This bFGF@Me-HA dressing was applied to a mouse with full-thickness excisional wounds to investigate its positive roles in wound repair owing to the complementary functions of HA with sustained release of bioactive bFGF. Compared with the single Me-HA and bFGF group, bFGF@Me-HA hydrogel dressings significantly enhanced wound healing with accelerated re-epithelialization, granulation formation, collagen, deposition and skin appendage regeneration. Further investigations showed significantly promoted cell proliferation and vascularization in the bFGF@Me-HA group, which was mediated by the upregulation of transforming growth factor-β (TGF-β) and vascular endothelial growth factor (VEGF) expressions. In conclusion, this bFGF@Me-HA hydrogel realized the optimization of simple ECM mimic dressing via introducing the bioactive effector, bFGF, and has the potential to be widely used as an effective bioactive ECM-based wound dressing in future wound care.

中文翻译：

通过持续释放 bFGF 促进皮肤伤口愈合的生物活性 ECM 模拟透明质酸敷料

成功的皮肤伤口再生需要修复细胞和细胞信号与细胞外基质 (ECM) 的协调，细胞外基质 (ECM) 是细胞功能不可或缺的机械和生物支持物，并在愈合过程中与各种细胞因子进行通讯。在这里，我们开发了一种可注射的生物活性伤口敷料，基于甲基丙烯酸化透明质酸 (Me-HA) 的水凝胶负载碱性成纤维细胞生长因子 (bFGF)，赋予敷料以模拟天然 ECM 的多效生物活性。将这种 bFGF@Me-HA 敷料应用于具有全层切除伤口的小鼠，以研究其在伤口修复中的积极作用，因为 HA 与生物活性 bFGF 的持续释放具有互补功能。与单一 Me-HA 和 bFGF 组相比，bFGF@Me-HA 水凝胶敷料通过加速上皮再形成、肉芽形成、胶原蛋白、沉积和皮肤附件再生显着促进伤口愈合。进一步的研究表明，bFGF@Me-HA 组显着促进细胞增殖和血管形成，这是由转化生长因子-β (TGF-β) 和血管内皮生长因子 (VEGF) 表达的上调介导的。总之，这种bFGF@Me-HA水凝胶通过引入生物活性效应物bFGF实现了简单ECM模拟敷料的优化，并有可能在未来的伤口护理中广泛用作有效的生物活性ECM伤口敷料。进一步的研究表明，bFGF@Me-HA 组显着促进细胞增殖和血管形成，这是由转化生长因子-β (TGF-β) 和血管内皮生长因子 (VEGF) 表达的上调介导的。总之，这种bFGF@Me-HA水凝胶通过引入生物活性效应物bFGF实现了简单ECM模拟敷料的优化，并有可能在未来的伤口护理中广泛用作有效的生物活性ECM伤口敷料。进一步的研究表明，bFGF@Me-HA 组显着促进细胞增殖和血管形成，这是由转化生长因子-β (TGF-β) 和血管内皮生长因子 (VEGF) 表达的上调介导的。总之，这种bFGF@Me-HA水凝胶通过引入生物活性效应物bFGF实现了简单ECM模拟敷料的优化，并有可能在未来的伤口护理中广泛用作有效的生物活性ECM伤口敷料。

更新日期：2020-04-02

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