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Membrane Stabilization of Poly(ethylene glycol)-b-polypeptide-g-trehalose Assists Cryopreservation of Red Blood Cells
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-04-02 , DOI: 10.1021/acsabm.0c00247 Bo Liu 1 , Lingyue Zhang 1 , Qifa Zhang 1 , Shuhui Gao 1 , Yunhui Zhao 1 , Lixia Ren 1 , Wenxiong Shi 2 , Xiaoyan Yuan 1
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-04-02 , DOI: 10.1021/acsabm.0c00247 Bo Liu 1 , Lingyue Zhang 1 , Qifa Zhang 1 , Shuhui Gao 1 , Yunhui Zhao 1 , Lixia Ren 1 , Wenxiong Shi 2 , Xiaoyan Yuan 1
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As alternative cryoprotectants of conventional organic glycerol, biocompatible synthetic glycopeptides that can assist cryopreservation of red blood cells (RBCs) are proposed in this study. A series of glycopeptides are synthesized via ring-opening polymerization of N-carboxyanhydrides of lysine, aspartic acid, and phenylalanine initiated by poly(ethylene glycol)-NH2 and followed by chemical tethering carboxylated trehalose to the pendant amino moieties. The synthetic glycopeptides demonstrate distinguished features of low cytotoxicity, low hemolysis, and cell membrane stabilization. The specifically designed glycopeptides enhance cryosurvival recovery of sheep RBCs up to 87.3 ± 0.3% at pH 6.0 and 86.5 ± 0.3% at pH 7.4 together with trehalose during cryopreservation. The synergistic cryopreservation of RBCs is achieved via membrane stabilization of the glycopeptide and ice recrystallization inhibition of trehalose during freezing and thawing. Molecular dynamics simulation indicates that the glycopeptides enhance membrane stabilization at −196 °C by tuning water diffusion. This work provides a potential option of highly efficient and glycerol-free RBC cryopreservation by combination of synthetic glycopeptides and trehalose, which would inspire design and utilization of different mechanisms for the effective cryopreservation of cells or tissues.
中文翻译：
聚（乙二醇）-b-多肽-g-海藻糖的膜稳定性有助于红细胞的冷冻保存
作为常规有机甘油的替代冷冻保护剂，本研究提出了有助于冷冻保存红细胞 (RBC) 的生物相容性合成糖肽。由聚乙二醇-NH 2引发的赖氨酸、天冬氨酸和苯丙氨酸的N-羧基酸酐开环聚合合成一系列糖肽然后将羧化海藻糖化学束缚到侧氨基部分。合成的糖肽表现出低细胞毒性、低溶血和细胞膜稳定的显着特征。专门设计的糖肽与海藻糖一起在冷冻保存期间将绵羊红细胞的冷冻存活率在 pH 6.0 时提高了 87.3 ± 0.3%，在 pH 7.4 时提高了 86.5 ± 0.3%。红细胞的协同冷冻保存是通过糖肽的膜稳定化和海藻糖在冷冻和解冻过程中的冰重结晶抑制来实现的。分子动力学模拟表明，糖肽通过调节水扩散增强了 -196 °C 下的膜稳定性。
更新日期：2020-04-02
中文翻译：
聚（乙二醇）-b-多肽-g-海藻糖的膜稳定性有助于红细胞的冷冻保存
作为常规有机甘油的替代冷冻保护剂，本研究提出了有助于冷冻保存红细胞 (RBC) 的生物相容性合成糖肽。由聚乙二醇-NH 2引发的赖氨酸、天冬氨酸和苯丙氨酸的N-羧基酸酐开环聚合合成一系列糖肽然后将羧化海藻糖化学束缚到侧氨基部分。合成的糖肽表现出低细胞毒性、低溶血和细胞膜稳定的显着特征。专门设计的糖肽与海藻糖一起在冷冻保存期间将绵羊红细胞的冷冻存活率在 pH 6.0 时提高了 87.3 ± 0.3%，在 pH 7.4 时提高了 86.5 ± 0.3%。红细胞的协同冷冻保存是通过糖肽的膜稳定化和海藻糖在冷冻和解冻过程中的冰重结晶抑制来实现的。分子动力学模拟表明，糖肽通过调节水扩散增强了 -196 °C 下的膜稳定性。
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