Reversible protein phosphorylation represents a key mechanism by which signals are transduced in eukaryotic cells. Dysregulated phosphorylation is also a hallmark of carcinogenesis and represents key drug targets in the precision medicine space. Thus, methods that preserve phosphoprotein integrity in the context of clinical tissue analyses are crucially important in cancer research. Here we investigated the impact of UV laser microdissection (UV LMD) and IR laser capture microdissection (IR LCM) on phosphoprotein abundance of key cancer signaling protein targets assessed by reverse-phase protein microarray (RPPA). Tumor epithelial cells from consecutive thin sections obtained from four high-grade serous ovarian cancers were harvested using either UV LMD or IR LCM methods. Phosphoprotein abundances for ten phosphoproteins that represent important drug targets were assessed by RPPA and revealed no significant differences in phosphoprotein integrity from those obtained using higher-energy UV versus the lower-energy IR laser methods.

中文翻译：

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基于紫外和红外的激光显微切割技术对激光显微切割-反相蛋白阵列工作流程中磷蛋白检测的影响。


可逆的蛋白质磷酸化代表了真核细胞中信号转导的关键机制。磷酸化失调也是致癌的一个标志，也是精准医学领域的关键药物靶点。因此，在临床组织分析中保持磷蛋白完整性的方法在癌症研究中至关重要。在这里，我们研究了紫外激光显微切割 (UV LMD) 和红外激光捕获显微切割 (IR LCM) 对通过反相蛋白微阵列 (RPPA) 评估的关键癌症信号蛋白靶点磷蛋白丰度的影响。使用 UV LMD 或 IR LCM 方法从四种高级别浆液性卵巢癌中获得的连续薄片中收获肿瘤上皮细胞。通过 RPPA 评估了代表重要药物靶标的 10 种磷蛋白的磷蛋白丰度，结果显示，使用高能 UV 与低能 IR 激光方法获得的磷蛋白完整性没有显着差异。